{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:35:33Z","timestamp":1771626933379,"version":"3.50.1"},"reference-count":50,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2001,8,1]]},"abstract":"<jats:p>This study provides new information about the relative importance of calcium mobilization and entry in the renal vascular response to adrenoceptor activation in afferent arterioles isolated from 7- to 8-wk-old Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Intracellular free calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) was measured in microdissected arterioles utilizing ratiometric photometry of fura 2 fluorescence. There was no significant strain difference in baseline [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. Norepinephrine (NE; 10<jats:sup>\u22126<\/jats:sup>and 10<jats:sup>\u22127<\/jats:sup>M) elicited immediate, sustained increases in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. The general temporal pattern of response to 10<jats:sup>\u22126<\/jats:sup>M NE consisted of an initial peak and a maintained plateau phase. The response to NE was partially blocked by nifedipine (10<jats:sup>\u22126<\/jats:sup>M) or 8-( N,N-diethylamino) octyl-3,4,5-trimetoxybenzoate (TMB-8; 10<jats:sup>\u22125<\/jats:sup>M). A calcium-free external solution abolished the sustained [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>plateau response to NE, with less influence on the peak response. In the absence of calcium entry, TMB-8 (10<jats:sup>\u22125<\/jats:sup>M) completely blocked the calcium response to NE in WKY but not SHR, suggesting strain differences in mobilization. A higher concentration of TMB-8 (10<jats:sup>\u22124<\/jats:sup>M), however, blocked all discernible mobilization in both strains. We conclude that there are differences in Ca<jats:sup>2+<\/jats:sup>handling in renal resistance vessels between young WKY and SHR with respect to mobilization stimulated by \u03b1-adrenoceptors. 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