{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T20:38:54Z","timestamp":1778272734056,"version":"3.51.4"},"reference-count":45,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2001,8,1]]},"abstract":"<jats:p> Apical H-K-ATPase in the cortical collecting duct (CCD) plays an important role in urinary acidification and K reabsorption. Our previous studies demonstrated that an H-K-ATPase mediates, in part, Rb reabsorption in rabbit CCD (Zhou X and Wingo CS. Am J Physiol Renal Fluid Electrolyte Physiol 263: F1134\u2013F1141, 1992). The purpose of these experiments was to examine using in vitro microperfused CCD from K-restricted rabbits 1) whether an acute increase in Pco <jats:sub>2<\/jats:sub> and, presumably, intracellular acidosis stimulate K absorptive flux; and 2) whether this stimulation was dependent on the presence of a functional H-K-ATPase. Rb reabsorption was significantly increased after exposure to 10% CO<jats:sub>2<\/jats:sub> in CCD, and this effect was persistent for the entire 10% CO<jats:sub>2<\/jats:sub> period, whereas 10 \u03bcM SCH-28080 in the perfusate totally abolished the stimulation of Rb reabsorption by 10% CO<jats:sub>2<\/jats:sub>. After stimulation of Rb reabsorption by 10% CO<jats:sub>2<\/jats:sub>, subsequent addition of 0.1 mM methazolamide, an inhibitor of carbonic anhydrase, failed to affect Rb reabsorption. However, simultaneous exposure to 10% CO<jats:sub>2<\/jats:sub> and methazolamide prevented the stimulation of Rb reabsorption. Treatment with the intracellular calcium chelator MAPTAM (0.5 \u03bcM) inhibited the stimulation of Rb reabsorption by 10% CO<jats:sub>2<\/jats:sub>. Similar inhibition was also observed in the presence of either a calmodulin inhibitor, W-7 (0.5 \u03bcM), or colchicine (0.5 mM), an inhibitor of tubulin polymerization. In time control studies, the perfusion time did not significantly affect Rb reabsorption. We conclude the following: 1) stimulation of Rb reabsorption on exposure to 10% CO<jats:sub>2<\/jats:sub> is dependent on the presence of a functional H-K-ATPase and appears to be regulated in part by the insertion of this enzyme into the apical plasma membrane by exocytosis; 2) insertion of H-K-ATPase requires changes in intracellular pH and needs a basal level of intracellular calcium concentration; and 3) H-K-ATPase insertion occurs by a microtubule-dependent process. <\/jats:p>","DOI":"10.1152\/ajprenal.2001.281.2.f366","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T14:08:31Z","timestamp":1514038111000},"page":"F366-F373","source":"Crossref","is-referenced-by-count":18,"title":["Increased CO<sub>2<\/sub> stimulates K\/Rb reabsorption mediated by H-K-ATPase in CCD of potassium-restricted rabbit"],"prefix":"10.1152","volume":"281","author":[{"given":"Xiaoming","family":"Zhou","sequence":"first","affiliation":[{"name":"Division of Nephrology, 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