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In the present study, we determined the pattern of electrogenic sodium-bicarbonate cotransporter protein expression in rat eye. For this purpose, pNBC1- and kNBC1-specific antibodies were generated and used to detect these NBC1 protein variants by immunoblotting and immunocytochemistry. pNBC1 is expressed in cornea, conjunctiva, lens, ciliary body, and retina, whereas the expression of kNBC1 is restricted to the conjunctiva. These results provide the first evidence for extrarenal kNBC1 protein expression. The data in this study will serve as a basis for understanding the molecular mechanisms responsible for abnormalities in ocular electrogenic sodium-bicarbonate cotransport in patients with mutations in the NBC1 gene.<\/jats:p>","DOI":"10.1152\/ajprenal.2001.281.5.f920","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T17:25:46Z","timestamp":1514049946000},"page":"F920-F935","source":"Crossref","is-referenced-by-count":77,"title":["Immunolocalization of electrogenic sodium-bicarbonate cotransporters pNBC1 and kNBC1 in the rat eye"],"prefix":"10.1152","volume":"281","author":[{"given":"Dean","family":"Bok","sequence":"first","affiliation":[{"name":"Jules Stein Eye Institute, Department of Neurobiology,"},{"name":"Brain Research Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Schibler","sequence":"additional","affiliation":[{"name":"Brain Research 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