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Periostin, initially identified in osteoblasts, is not expressed in normal adult kidneys but is expressed transiently during renal development. We found periostin in cyst-lining cells in situ in the extracellular matrix adjacent to the cysts and within cyst fluid. ADPKD cells secreted periostin across luminal and basolateral plasma membranes. Periostin increased proliferation of cyst epithelial cells 27.9 \u00b1 3.1% ( P &lt; 0.001) above baseline and augmented in vitro cyst growth but did not affect proliferation of normal renal cells. Expression of \u03b1<jats:sub>V<\/jats:sub>-integrin, a periostin receptor, was ninefold higher in ADPKD cells compared with NHK cells, and antibodies that block \u03b1<jats:sub>V<\/jats:sub>-integrin inhibited periostin-induced cell proliferation. We conclude that periostin is a novel autocrine mitogen secreted by mural epithelial cells with the potential to accelerate cyst growth and promote interstitial remodeling in ADPKD.<\/jats:p>","DOI":"10.1152\/ajprenal.90266.2008","type":"journal-article","created":{"date-parts":[[2008,8,28]],"date-time":"2008-08-28T00:44:32Z","timestamp":1219884272000},"page":"F1463-F1471","source":"Crossref","is-referenced-by-count":75,"title":["Periostin induces proliferation of human autosomal dominant polycystic kidney cells through \u03b1<sub>V<\/sub>-integrin receptor"],"prefix":"10.1152","volume":"295","author":[{"given":"Darren P.","family":"Wallace","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan T.","family":"Quante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gail A.","family":"Reif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Nivens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhana","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott J.","family":"Hempson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Blanco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamio","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(04)00081-9"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00843.x"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00768.x"},{"key":"R4","unstructured":"Calvet JP.Molecular genetics of polycystic kidney disease.J Nephrol11: 24\u201334, 1998."},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.1.81"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00065.x"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206347"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1980.43"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402700208"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.304"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.4161\/cc.4.11.2185"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1038\/nm935"},{"key":"R13","unstructured":"Gillan L, Matei D, Fishman DA, Gerbin CS, Karlan BY, Chang DD.Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility.Cancer Res62: 5358\u20135364, 2002."},{"key":"R14","unstructured":"Grantham JJ.1992 Homer Smith Award. 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