{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T22:14:33Z","timestamp":1771971273700,"version":"3.50.1"},"reference-count":26,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p>NKCC1 is a widely expressed isoform of the Na-2Cl-K cotransporter that mediates several direct and indirect vascular effects and regulates expression and release of renin. In this study, we used NKCC1-deficient (NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>) and wild-type (WT) mice to assess day\/night differences of blood pressure (BP), locomotor activity, and renin release and to study the effects of high (8%) or low (0.03%) dietary NaCl intake on BP, activity, and the renin\/aldosterone system. On a standard diet, 24-h mean arterial blood pressure (MAP) and heart rate determined by radiotelemetry, and their day\/night differences, were not different in NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>and WT mice. Spontaneous and wheel-running activities in the active night phase were lower in NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>than WT mice. In NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>mice on a high-NaCl diet, MAP increased by 10 mmHg in the night without changes in heart rate. In contrast, there was no salt-dependent blood pressure change in WT mice. MAP reductions by hydralazine (1 mg\/kg) or isoproterenol (10 \u03bcg\/mouse) were significantly greater in NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>than WT mice. Plasma renin (PRC; ng ANG I\u00b7ml<jats:sup>\u22121<\/jats:sup>\u00b7h<jats:sup>\u22121<\/jats:sup>) and aldosterone (aldo; pg\/ml) concentrations were higher in NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>than WT mice (PRC: 3,745 \u00b1 377 vs. 1,245 \u00b1 364; aldo: 763 \u00b1 136 vs. 327 \u00b1 98). Hyperreninism and hyperaldosteronism were found in NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>mice during both day and night. High Na suppressed PRC and aldosterone in both NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>and WT mice, whereas a low-Na diet increased PRC and aldosterone in WT but not NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>mice. We conclude that 24-h MAP and MAP circadian rhythms do not differ between NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>and WT mice on a standard diet, probably reflecting a balance between anti- and prohypertensive factors, but that blood pressure of NKCC1<jats:sup>\u2212\/\u2212<\/jats:sup>mice is more sensitive to increases and decreases of Na intake.<\/jats:p>","DOI":"10.1152\/ajprenal.90392.2008","type":"journal-article","created":{"date-parts":[[2008,10,9]],"date-time":"2008-10-09T11:57:43Z","timestamp":1223553463000},"page":"F1230-F1238","source":"Crossref","is-referenced-by-count":46,"title":["Salt sensitivity of blood pressure in NKCC1-deficient mice"],"prefix":"10.1152","volume":"295","author":[{"given":"Soo Mi","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Eisner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Faulhaber-Walter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane","family":"Mizel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Wall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josephine P.","family":"Briggs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jurgen","family":"Schnermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.6.C1383"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.2001.5.2.89"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.35.2.e1"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00455.2004"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(00)00024-9"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1038\/9713"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.5.2.137"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00134-4"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2007060721"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.38.26946"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01402.2006"},{"key":"R12","doi-asserted-by":"crossref","unstructured":"Ginns SM, Knepper MA, Ecelbarger CA, Terris J, He X, Coleman RA, Wade JB.Immunolocalization of the secretory isoform of Na-K-Cl cotransporter in rat renal intercalated cells.J Am Soc Nephrol7: 2533\u20132542, 1996.","DOI":"10.1681\/ASN.V7122533"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000060866.69947.D1"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.62.1.515"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.53.5.630"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118844"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00074.2008"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(81)90150-3"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00083.2002"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00073.2007"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8553"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.1.C173"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.1.211"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.041897"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.5.F913"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00309.2005"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.90392.2008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T05:50:14Z","timestamp":1631944214000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.90392.2008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1152\/ajprenal.90392.2008"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.90392.2008","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10]]}}}