{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T02:07:43Z","timestamp":1785550063151,"version":"3.56.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1976,10,1]]},"abstract":"<jats:p> We describe a method for perfusion of a working isolated rat heart with washed erythrocytes suspended in a Krebs-Henseleit bicarbonate buffer containing bovine albumin (fraction V). With washed pig red cells, as hematocritwas varied between 0 and 40%, coronary flow (CF), aortic flow (AF), external work (W), and myocardial oxygen consumption (MVO2) were measured. Hemodynamic data at a hematocrit of 30% (CF = 5.4 +\/- 0.7 ml\/min per g, AF = 75 +\/- 8 ml\/min per g) were identical with those reported for the intact animal.Coronary sinus PO2 was highest with a red cell-free perfusate suggesting that coronary flow is partially shunted. Human red cells obtained from bankedblood, were tried also with success. With careful filtration, the preparation is stable for 2 h and well suited for study of the dynamics of myocardial oxygen delivery. <\/jats:p>","DOI":"10.1152\/jappl.1976.41.4.603","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:13:59Z","timestamp":1514157239000},"page":"603-607","source":"Crossref","is-referenced-by-count":44,"title":["Blood-perfused working isolated rat heart"],"prefix":"10.1152","volume":"41","author":[{"given":"M. A.","family":"Duvelleroy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Duruble","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Teisseire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Droulez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Cain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1976.41.4.603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:13:53Z","timestamp":1567988033000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1976.41.4.603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1976,10,1]]}},"alternative-id":["10.1152\/jappl.1976.41.4.603"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1976.41.4.603","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,10,1]]}}}