{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:20:37Z","timestamp":1773292837028,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1987,4,1]]},"abstract":"<jats:p> The polycation protamine sulfate increases microvascular permeability in the kidney by reducing glomerular charge. We have exposed the pulmonary vasculature to protamine sulfate to determine whether electrical charges play a role in protein permeability in lung vascular beds. In anephric rats, protamine sulfate increased hematocrit approximately 25%. With protamine sulfate doses of 0.08 and 0.04 mg\/g body wt, lung blood-free wet-to-dry weight ratios were increased (5.24 +\/- 0.8 and 4.89 +\/- 0.7) compared with control (3.85 +\/- 0.3) (P less than 0.05). In isolated, ventilated, and perfused lungs 0.04 mg\/g body wt protamine sulfate increased pulmonary arterial pressure from 5.2 +\/- 1.4 to 16.3 +\/- 3.9 mmHg (P less than 0.01). These lungs gained weight and lung wet-to-dry weight ratios were significantly increased (15.33 +\/- 4.26 compared with 6.04 +\/- 0.24 for control lungs). Poly-L-lysine, another polycation, also caused significant increases in pulmonary arterial pressure, lung weight, and lung wet-to-dry weight ratios. The addition of diphenhydramine to the perfusate 10 min before the addition of protamine sulfate did not prevent these changes. Heparin (90 U\/mg protamine sulfate) reversed the abnormalities. Pulmonary arterial pressure (7.0 +\/- 1.1 mmHg) was not significantly different from the control value, lung weight did not increase, and the lung wet-to-dry weight ratio was 6.24 +\/- 0.23 (P greater than 0.05). We conclude that polycations have a significant effect on pulmonary vascular resistance and perhaps on permeability. <\/jats:p>","DOI":"10.1152\/jappl.1987.62.4.1363","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T12:15:10Z","timestamp":1513858510000},"page":"1363-1367","source":"Crossref","is-referenced-by-count":15,"title":["Protamine sulfate causes pulmonary hypertension and edema in isolated rat lungs"],"prefix":"10.1152","volume":"62","author":[{"given":"R. P.","family":"Fairman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. N.","family":"Sessler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bierman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. L.","family":"Glauser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1987.62.4.1363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T15:07:28Z","timestamp":1567955248000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1987.62.4.1363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4,1]]}},"alternative-id":["10.1152\/jappl.1987.62.4.1363"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1987.62.4.1363","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4,1]]}}}