{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:48:47Z","timestamp":1762102127967},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1987,5,1]]},"abstract":"<jats:p> The relationships between pHi (intracellular pH) and phosphate compounds were evaluated by nuclear magnetic resonance (NMR) in normo-, hypo-, and hypercapnia, obtained by changing fractional inspired concentration of CO2 in dogs anesthetized with 0.75% isoflurane and 66% N2O. Phosphocreatine (PCr) fell by 2.02 mM and Pi (inorganic phosphate) rose by 1.92 mM due to pHi shift from 7.10 to 6.83 during hypercapnia. The stoichiometric coefficient was 1.05 (r2 = 0.78) on log PCr\/Cr against pHi, showing minimum change of ADP\/ATP and equilibrium of creatine kinase in the pH range of 6.7 to 7.25. [ADP] varied from 21.6 +\/- 4.1 microM in control (pHi = 7.10) to 26.8 +\/- 6.3 microM in hypercapnia (pHi = 6.83) and 24.0 +\/- 6.8 microM in hypocapnia (pHi = 7.17). ATP\/ADP X Pi decreased from 66.4 +\/- 17.1 mM-1 during normocapnia to 25.8 +\/- 6.3 mM-1 in hypercapnia. The ADP values are near the in vitro Km; thus ADP is the main controller. The velocity of oxidative metabolism (V) in relation to its maximum (Vmax) as calculated by a steady-state Michaelis-Menten formulation is approximately 50% in normocapnia. In acidosis (pH 6.7) and alkalosis (pH 7.25), V\/Vmax is 10% higher than the normocapnic brain. This increase of V\/Vmax is required to maintain cellular homeostasis of energy metabolism in the face of either inhibition at extremes of pH or higher ATPase activity. <\/jats:p>","DOI":"10.1152\/jappl.1987.62.5.2094","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T12:05:06Z","timestamp":1513857906000},"page":"2094-2102","source":"Crossref","is-referenced-by-count":57,"title":["Relationship between intracellular pH and energy metabolism in dog brain as measured by 31P-NMR"],"prefix":"10.1152","volume":"62","author":[{"given":"S.","family":"Nioka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Chance","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hilberman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. V.","family":"Subramanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"J. S.","family":"Leigh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. L.","family":"Veech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E.","family":"Forster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1987.62.5.2094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T15:06:30Z","timestamp":1567955190000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1987.62.5.2094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1987,5,1]]}},"alternative-id":["10.1152\/jappl.1987.62.5.2094"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1987.62.5.2094","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,5,1]]}}}