{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T16:01:24Z","timestamp":1772899284840,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1987,12,1]]},"abstract":"<jats:p> We hypothesized that toxic O2 radicals might be important mediators of endotoxin-induced acute respiratory failure in pigs. As a relatively specific scavenger of .OH, we infused dimethylthiourea (DMTU, 1 g\/kg) before endotoxemia. Escherichia coli endotoxin (055-B5) was infused intravenously into anesthetized 10- to 14-wk-old pigs at 5 micrograms\/kg the 1st h, followed by 2 micrograms.kg-1.h-1 for 3.5 h. During phase 1 (i.e., 0\u20132 h) and phase 2 (i.e., 2\u20134.5 h), endotoxin decreased cardiac index (CI) and increased mean pulmonary arterial pressure (Ppa), pulmonary vascular resistance (PVR), alveolar-arterial O2 gradient (AaDo2), and hematocrit (Hct). Endotoxemia also caused leukopenia and increased the postmortem bronchoalveolar lavage fluid (BALF) albumin concentration and wet weight-to-dry weight ratio of bloodless lung. Dimethylthiourea did not significantly modify the phase 1 response. However, during phase 2, DMTU attenuated the endotoxin-induced decrease in CI and increases in Ppa, PVR, Hct, AaDo2, lung water, and BALF albumin concentration. In separate groups of endotoxin- and DMTU + endotoxin-treated pigs, lung microvascular hydrostatic pressure was increased to approximately 16 Torr (by fluid overload) to assess alveolar-capillary membrane permeability. Under these conditions, DMTU markedly attenuated the endotoxin-induced increase in alveolar-capillary membrane permeability. Under these conditions, DMTU markedly attenuated the endotoxin-induced induced increase in alveolar-capillary membrane permeability. We conclude that .OH (and possibly H2O2) significantly contributes to endotoxin-induced lung injury in anesthetized pigs. <\/jats:p>","DOI":"10.1152\/jappl.1987.63.6.2426","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T21:09:36Z","timestamp":1513890576000},"page":"2426-2432","source":"Crossref","is-referenced-by-count":29,"title":["Dimethylthiourea attenuates endotoxin-induced acute respiratory failure in pigs"],"prefix":"10.1152","volume":"63","author":[{"given":"N. C.","family":"Olson","sequence":"first","affiliation":[{"name":"Department of Anatomy, Physiological Sciences and Radiology, School of Veterinary Medicine, North Carolina State University, Raleigh 27606."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiological Sciences and Radiology, School of Veterinary Medicine, North Carolina State University, Raleigh 27606."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. K.","family":"Grizzle","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiological Sciences and Radiology, School of Veterinary Medicine, North Carolina State University, Raleigh 27606."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1987.63.6.2426","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:06:27Z","timestamp":1567958787000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1987.63.6.2426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1987,12,1]]}},"alternative-id":["10.1152\/jappl.1987.63.6.2426"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1987.63.6.2426","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,12,1]]}}}