{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:38:12Z","timestamp":1757705892052},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1988,10,1]]},"abstract":"<jats:p> Intact erythrocytes from subjects with deficiency of blood carbonic anhydrase (CA) II and from normal subjects were assayed for enzyme activity by use of an 18O exchange technique in a solution containing 25 mM (CO2 + NaHCO3) plus 125 mM NaCl. At 25 degrees C and pH 7.4, the catalyzed reaction velocity was 0.32 +\/- 0.04 M\/s for the CA II-deficient and 1.60 +\/- 0.12 M\/s for the normal cells, a ratio of 1:5. Under the same conditions at 37 degrees C the relative difference between the CA II-deficient and normal cells was much less: the velocity for the CA II-deficient cells was 0.84 +\/- 0.07 M\/s and for the normal cells 1.60 +\/- 0.32 M\/s, a ratio of 1:1.9. Results were comparable for the hemolysates with the NaHCO3 reduced to 85 mM (the corresponding intracellular concentration): at 25 degrees C CA II-deficient cells had a velocity of 0.36 +\/- 0.01 M\/s compared with 1.12 +\/- 0.04 M\/s for the normal cells, a ratio of 1:3.1. At 37 degrees C again the relative difference between hemolysates from CA II normal and deficient cells was much less: the CA II-deficient cells had a reaction velocity of 1.17 +\/- 0.22 M\/s vs. 2.60 +\/- 0.36 M\/s for the normal cells, a ratio of 1:2.2. The greater fractional reduction of enzyme velocity of CA II-deficient cells at 25 degrees C compared with 37 degrees C appears to be explained by a greater chloride inhibition of the presumed CA I at the lower temperature.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/jappl.1988.65.4.1472","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T22:37:24Z","timestamp":1513895844000},"page":"1472-1480","source":"Crossref","is-referenced-by-count":19,"title":["Carbonic anhydrase activity of intact carbonic anhydrase II-deficient human erythrocytes"],"prefix":"10.1152","volume":"65","author":[{"given":"S. J.","family":"Dodgson","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"2nd","given":"R. E.","family":"Forster","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. S.","family":"Sly","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E.","family":"Tashian","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1988.65.4.1472","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:16:08Z","timestamp":1567959368000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1988.65.4.1472"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1988,10,1]]}},"alternative-id":["10.1152\/jappl.1988.65.4.1472"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1988.65.4.1472","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,10,1]]}}}