{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:47:23Z","timestamp":1760550443864},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1991,5,1]]},"abstract":"<jats:p> Full methacholine dose-response curves were performed on anesthetized tracheostomized Fischer 344 adult rats treated neonatally with capsaicin (50 mg\/kg) or with vehicle alone. Capsaicin, the hot extract of pepper, releases substance P (SP) from nonmyelinated sensory nerve endings and causes acute bronchoconstriction and airway microvascular leakiness. Chronic treatment with capsaicin leads to depletion of SP and other tachykinins from afferent C-fibers and can therefore be used as a tool to investigate the contribution of SP innervation to airway responses. The rats (9 controls and 6 treated with capsaicin) were paralyzed with succinylcholine and mechanically ventilated at a constant tidal volume and frequency. Airway resistance (RL) and dynamic compliance (Cdyn) were determined at each dose of methacholine from measurements of volume, flow, and transpulmonary pressure. Capsaicin-treated rats were found to have a significantly reduced baseline RL [0.150 +\/- 0.039 (SD) vs. 0.225 +\/- 0.050 cmH2O.ml-1.s, P = 0.009] and a correspondingly significantly elevated Cdyn (0.371 +\/- 0.084 vs. 0.268 +\/- 0.053 ml\/cmH2O, P = 0.012). There was no significant difference in sensitivity to methacholine, but the maximal response to methacholine was significantly greater in the capsaicin-treated rats. In terms of RL, the maximal response for capsaicin-treated rats was 6.03 x baseline +\/- 0.98 vs. 4.30 x baseline +\/- 1.80 (P = 0.05) for controls, and for Cdyn changes the maximal decrease was 5.75 x baseline +\/- 1.22 vs. 3.83 +\/- 0.69 (P = 0.002). The observed differences in RL and Cdyn coupled with the differences in maximal responses can be attributed to the selective destruction of a subpopulation of pulmonary afferent C-fibers.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/jappl.1991.70.5.2087","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T02:01:37Z","timestamp":1513908097000},"page":"2087-2094","source":"Crossref","is-referenced-by-count":4,"title":["Neonatal capsaicin treatment alters basal pulmonary mechanics and response to methacholine in F344 rats"],"prefix":"10.1152","volume":"70","author":[{"given":"E. M.","family":"App","sequence":"first","affiliation":[{"name":"Pulmonary Defense Group, University of Alberta, Edmonton, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. T.","family":"De Sanctis","sequence":"additional","affiliation":[{"name":"Pulmonary Defense Group, University of Alberta, Edmonton, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. H.","family":"Green","sequence":"additional","affiliation":[{"name":"Pulmonary Defense Group, University of Alberta, Edmonton, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. C.","family":"Hulbert","sequence":"additional","affiliation":[{"name":"Pulmonary Defense Group, University of Alberta, Edmonton, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. K.","family":"Rubin","sequence":"additional","affiliation":[{"name":"Pulmonary Defense Group, University of Alberta, Edmonton, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Remmers","sequence":"additional","affiliation":[{"name":"Pulmonary Defense Group, University of Alberta, Edmonton, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"King","sequence":"additional","affiliation":[{"name":"Pulmonary Defense Group, University of Alberta, Edmonton, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1991.70.5.2087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:49:20Z","timestamp":1567961360000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1991.70.5.2087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,5,1]]}},"alternative-id":["10.1152\/jappl.1991.70.5.2087"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1991.70.5.2087","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,5,1]]}}}