{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:39:18Z","timestamp":1773459558393,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1993,5,1]]},"abstract":"<jats:p> Glucose consumption in the rabbit carotid body was studied in vitro by measuring phosphorylation rates of tracer concentrations of 2-[3H]deoxy-glucose. The rate of glucose consumption measured in 100% O2-equilibrated modified Tyrode medium was 61 nmol.g tissue-1 x min-1 and was linear for up to 30 min. Incubation of carotid bodies for 5 or 10 min in moderately hypoxic solution (20% O2\u201380% N2) resulted in a 44% increase in the rate of glucose consumption. The glucose consumption of the nodose ganglion was not affected during similar incubation with low-O2 medium. High-resolution autoradiography of freeze-dried tissues revealed that the type I parenchymal cells are the principal site of glucose consumption in both 100% O2- and 20% O2-incubated carotid bodies. This metabolic response of the carotid body to hypoxia was not secondary to neurotransmitter release, because similar elevations in glucose utilization were observed with low-O2 medium containing zero Ca2+, a condition in which the release of neurotransmitters from type I cells is inhibited. Lowering the pH of the incubation medium from 7.4 to 7 or 6.8 markedly reduced the rate of glucose utilization by both the carotid body and the nodose ganglion. Ouabain (2 x 10(-4) and 1 x 10(-3) M) reduced by 20% the glucose consumption of carotid bodies incubated in 100% O2-equilibrated solution and abolished the metabolic response produced by low-O2 medium. The results suggest that the utilization of metabolic energy is an integral component of the chemoreceptor response to hypoxia. <\/jats:p>","DOI":"10.1152\/jappl.1993.74.5.2387","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T18:27:47Z","timestamp":1513880867000},"page":"2387-2393","source":"Crossref","is-referenced-by-count":25,"title":["Effect of low O2 on glucose uptake in rabbit carotid body"],"prefix":"10.1152","volume":"74","author":[{"given":"A.","family":"Obeso","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Rigual","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Dinger","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Fidone","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1993.74.5.2387","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T12:25:31Z","timestamp":1567945531000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1993.74.5.2387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,5,1]]}},"alternative-id":["10.1152\/jappl.1993.74.5.2387"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1993.74.5.2387","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5,1]]}}}