{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T07:09:37Z","timestamp":1772003377759,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:p> To evaluate the adenosine systems ability to reverse the endothelial damage produced by ischemia and reperfusion (I\/R), we studied several different selective adenosine-receptor agonists and antagonists, a protein kinase A inhibitor, and a beta-adrenoreceptor antagonist in isolated buffer-perfused rat lungs. I\/R (45 min\/105 min) produced a sixfold increase in endothelial permeability as measured by the capillary filtration coefficient. Both a selective A2-receptor agonist (CGS-21680, 300 nM) and a beta-receptor agonist (isoproterenol, 10 microM) reversed the increased microvascular permeability. A nonselective adenosine-receptor antagonist (SPT, 20 microM) and a selective A1-receptor antagonist (DPCPX, 10 nM) had no effect on increased microvascular permeability. Also, isoproterenol and CGS-21680 reversed the damage being introduced after a selective A1-receptor agonist (CCPA, 100 nM). The nonspecific adenosine A1- and A2-receptor agonist NECA (12 nM) appeared to desensitize the A2 receptors and a protein kinase A inhibitor, adenosine-3\u2032,5\u2032-cyclic monophosphothioate (Rp-cAMPS, 100 microM), blocked the reversal of endothelial damage by isoproterenol or A2-receptor agonist. Propranolol (100 microM) blocked the effect of isoproterenol but not the effect of CGS-21680. From this study we conclude that A2-receptor activation reverses endothelial damage associated with I\/R by a mechanism independent of beta-receptors or Gi protein. However, a protein kinase A-3\u2032,5\u2032,-cyclic adenosine monophosphate pathway is activated by both the adenosine systems and beta-receptor activation. <\/jats:p>","DOI":"10.1152\/jappl.1995.78.3.990","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T11:28:51Z","timestamp":1513942131000},"page":"990-996","source":"Crossref","is-referenced-by-count":31,"title":["Adenosine A2 receptors reverse ischemia-reperfusion lung injury independent of beta-receptors"],"prefix":"10.1152","volume":"78","author":[{"given":"P. L.","family":"Khimenko","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. M.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. W.","family":"Hill","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. S.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Coleman","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Rizzo","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. E.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1995.78.3.990","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T18:02:20Z","timestamp":1567965740000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1995.78.3.990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3,1]]}},"alternative-id":["10.1152\/jappl.1995.78.3.990"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1995.78.3.990","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3,1]]}}}