{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T05:57:36Z","timestamp":1656050256539},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1996,7,1]]},"abstract":"<jats:p> Magnesium ions (Mg2+) play a crucial role in the activation and synthesis of guanine nucleotide-binding proteins (G proteins). However, there is no information about the influence of in vivo magnesium deficiency on the function and levels of G proteins. This study was done to investigate whether dietary magnesium deficiency alters function and levels of the two major myocardial G proteins, Gi alpha and Gs alpha. Severe hypomagnesemia and a significant reduction of myocardial magnesium occurred in rats fed a magnesium-deficient diet for 6 wk vs. rats fed a normal-magnesium diet (control). The magnesium-deficient rats developed focal myocardial lesions but their cardiac function was not impaired. Myocardial immunodetectable Gs alpha and Gi alpha levels of magnesium-deficient rats did not differ from control (Gs alpha: 2.39 +\/- 0.52 vs. 2.76 +\/- 0.72 arbitrary units\/microgram protein, P &gt; 0.05; Gi alpha: 1.60 +\/- 0.52 vs. 1.89 +\/- 0.30 arbitrary units\/microgram protein, P &gt; 0.05). Similarly, the function of Gs alpha and Gi alpha estimated by basal and ligand-stimulated adenylyl cyclase activity was not significantly different between the two groups of animals. The results show that dietary-derived magnesium deficiency sufficient to produce severe hypomagnesemia does not produce any significant change in levels or function of myocardial G proteins. <\/jats:p>","DOI":"10.1152\/jappl.1996.81.1.335","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T06:31:41Z","timestamp":1513924301000},"page":"335-340","source":"Crossref","is-referenced-by-count":2,"title":["Severe dietary magnesium deficiency does not alter levels and function of myocardial Gs alpha and Gi alpha"],"prefix":"10.1152","volume":"81","author":[{"given":"B.","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Texas Tech University Health Sciences Center, Texas Tech University, Lubbock 79430, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Heavner","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Texas Tech University Health Sciences Center, Texas Tech University, Lubbock 79430, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Spallholz","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Texas Tech University Health Sciences Center, Texas Tech University, Lubbock 79430, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. M.","family":"Boylan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Texas Tech University Health Sciences Center, Texas Tech University, Lubbock 79430, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.1996.81.1.335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T17:15:11Z","timestamp":1567962911000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.1996.81.1.335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,7,1]]}},"alternative-id":["10.1152\/jappl.1996.81.1.335"],"URL":"https:\/\/doi.org\/10.1152\/jappl.1996.81.1.335","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7,1]]}}}