{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:38:21Z","timestamp":1791398301392,"version":"4.3.3"},"reference-count":27,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1996,11,1]]},"abstract":"<jats:p> Nattie, Eugene E., and Aihua Li. Central chemoreception in the region of the ventral respiratory group in the rat. J. Appl. Physiol. 81(5): 1987\u20131995, 1996.\u2014We injected acetazolamide (AZ; 5 \u00d7 10<jats:sup>\u22126<\/jats:sup> M, 1 nl) into the region of the ventral respiratory group (VRG) of anesthetized paralyzed ventilated rats. Control injections (mock cerebrospinal fluid, n = 6, or the inactive AZ analogue 2-acetylamino-1,3,4-thiadiazole-5-sulfon- t-butylamide, n = 6) did not increase the integrated phrenic neurogram [phrenic nerve amplitude (PNA)]. The AZ injections produced a focal region of tissue acidosis with a radius &lt; 300\u2013400 \u03bcm and are used as a probe for sites of central chemosensitivity. Injection location is determined by anatomic analysis. Of 22 VRG injections of AZ, 14 increased the amplitude of the PNA over 15\u201390 min; 8 had no effect. In 17 cases, we measured medullary tissue pH at the injection center and\/or at a distant site and reaffirmed the size of the acidotic region produced by such small AZ injections. Of injections with pH electrodes within 300\u2013400 \u03bcm of the injection center, all responders showed an acid pH; three nonresponders showed an acid pH, and one an alkaline pH. In a subgroup of five rats, at VRG sites with known respiratory effects identified by prior glutamate injection (10 nl, 100 mM), all subsequent AZ injections produced a PNA response. Simultaneous measurement of PNA and tissue pH responses at the injection center of eight rats did not show a uniform correlation in time; initially, both changed with a similar time course, but PNA recovered more quickly. We conclude that 1) the region of the VRG contains sites of ventilatory chemoreception, 2) ineffective AZ injections do produce a tissue acidosis but at sites with minimal impact on breathing, and 3) tissue pH does not uniquely represent the chemoreceptor stimulus. <\/jats:p>","DOI":"10.1152\/jappl.1996.81.5.1987","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T02:12:53Z","timestamp":1513908773000},"page":"1987-1995","source":"Crossref","is-referenced-by-count":73,"title":["Central chemoreception in the region of the ventral  respiratory group in the rat"],"prefix":"10.1152","volume":"81","author":[{"given":"Eugene E.","family":"Nattie","sequence":"first","affiliation":[{"name":"Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756-0001"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756-0001"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.80.1.108"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1988.65.1.422"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018902"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90191-A"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018760"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.75.1.5"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90363-9"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(94)90117-1"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90085-6"},{"key":"B11","first-page":"828","volume":"18","author":"Haxhiu M. 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