{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:31:55Z","timestamp":1792665115917,"version":"4.5.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:p>The objectives of this research were to determine the contribution of excitation-contraction (E-C) coupling failure to the decrement in maximal isometric tetanic force (P<jats:sub>o<\/jats:sub>) in mouse extensor digitorum longus (EDL) muscles after eccentric contractions and to elucidate possible mechanisms. The left anterior crural muscles of female ICR mice ( n = 164) were injured in vivo with 150 eccentric contractions. P<jats:sub>o<\/jats:sub>, caffeine-, 4-chloro- m-cresol-, and K<jats:sup>+<\/jats:sup>-induced contracture forces, sarcoplasmic reticulum (SR) Ca<jats:sup>2+<\/jats:sup>release and uptake rates, and intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) were then measured in vitro in injured and contralateral control EDL muscles at various times after injury up to 14 days. On the basis of the disproportional reduction in P<jats:sub>o<\/jats:sub>(\u223c51%) compared with caffeine-induced force (\u223c11\u201321%), we estimate that E-C coupling failure can explain 57\u201375% of the P<jats:sub>o<\/jats:sub>decrement from 0 to 5 days postinjury. Comparable reductions in P<jats:sub>o<\/jats:sub>and K<jats:sup>+<\/jats:sup>-induced force (51%), and minor reductions (0\u20136%) in the maximal SR Ca<jats:sup>2+<\/jats:sup>release rate, suggest that the E-C coupling defect site is located at the t tubule-SR interface immediately after injury. Confocal laser scanning microscopy indicated that resting [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was elevated and peak tetanic [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was reduced, whereas peak 4-chloro- m-cresol-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was unchanged immediately after injury. By 3 days postinjury, 4-chloro- m-cresol-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>became depressed, probably because of decreased SR Ca<jats:sup>2+<\/jats:sup>release and uptake rates (17\u201331%). These data indicate that the decrease in P<jats:sub>o<\/jats:sub>during the first several days after injury primarily stems from a failure in the E-C coupling process.<\/jats:p>","DOI":"10.1152\/jappl.1998.85.1.58","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T20:06:46Z","timestamp":1513973206000},"page":"58-67","source":"Crossref","is-referenced-by-count":210,"title":["E-C coupling failure in mouse EDL muscle after in vivo eccentric contractions"],"prefix":"10.1152","volume":"85","author":[{"given":"Christopher P.","family":"Ingalls","sequence":"first","affiliation":[{"name":"Muscle Biology Laboratory, Texas A&amp;M University, College Station, Texas 77843-4243; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon L.","family":"Warren","sequence":"additional","affiliation":[{"name":"Muscle Biology Laboratory, Texas A&amp;M University, College Station, Texas 77843-4243; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay H.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute, Blacksburg, Virginia 24061-0430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher W.","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute, Blacksburg, Virginia 24061-0430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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