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For this we exposed mast cell-deficient WBB6F<jats:sub>1<\/jats:sub>- kit<jats:sup>W<\/jats:sup>\/ kit<jats:sup>W-v<\/jats:sup>( kit<jats:sup>W<\/jats:sup>\/ kit<jats:sup>W-v<\/jats:sup>) mice and the congenic normal WBB6F<jats:sub>1<\/jats:sub>(+\/+) mice to air or to 1 or 3 parts\/million O<jats:sub>3<\/jats:sub>for 4 h and studied them at different intervals from 4 to 72 h later. We found evidence of O<jats:sub>3<\/jats:sub>-induced cutaneous, as well as bronchial, mast cell degranulation. Polymorphonuclear cell influx into the pulmonary parenchyma was observed after exposure to 1 part\/milllion O<jats:sub>3<\/jats:sub>only in mice that possessed mast cells. Airway hyperresponsiveness to intravenous methacholine measured in vivo under pentobarbital anesthesia was observed in both kit<jats:sup>W<\/jats:sup>\/ kit<jats:sup>W-v<\/jats:sup>and +\/+ mice after exposure to O<jats:sub>3<\/jats:sub>. Thus, although mast cells are activated in vivo by O<jats:sub>3<\/jats:sub>and participate in O<jats:sub>3<\/jats:sub>-induced polymorphonuclear cell infiltration into the pulmonary parenchyma, they do not participate detectably in the development of O<jats:sub>3<\/jats:sub>-induced airway hyperresponsiveness in mice.<\/jats:p>","DOI":"10.1152\/jappl.1999.86.1.202","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T13:03:36Z","timestamp":1513947816000},"page":"202-210","source":"Crossref","is-referenced-by-count":14,"title":["Mast cell activation is not required for induction of airway hyperresponsiveness by ozone in mice"],"prefix":"10.1152","volume":"86","author":[{"given":"N.","family":"Noviski","sequence":"first","affiliation":[{"name":"Children\u2019s Service, Neonatal and Pediatric Intensive Care Units, Massachusetts General Hospital, Boston 02114;"},{"name":"Combined Program in Pulmonary and Critical Care Medicine, Beth Israel Hospital and Brigham and Women\u2019s Hospital, Boston 02115;"},{"name":"Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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