{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T04:40:02Z","timestamp":1751172002733,"version":"3.41.0"},"reference-count":26,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[1999,10,1]]},"abstract":"<jats:p>Neutral endopeptidase (NEP) is one of the major endopeptidases responsible for the inactivation of substance P in the carotid body, a neurotransmitter shown to be important in the transduction of hypoxic stimuli. Ventilatory responses to acute hypoxia were measured by indirect plethysmography in unanesthetized, unrestrained wild-type mice and in mice in which the NEP gene was deleted (NEP -\/-). Ventilation was measured while the animals breathed room air: 12% O<jats:sub>2<\/jats:sub>in N<jats:sub>2<\/jats:sub>and 8% O<jats:sub>2<\/jats:sub>in N<jats:sub>2<\/jats:sub>. Deletion of the NEP gene caused marked alterations in both the magnitude and composition of the hypoxic ventilatory response to both 8% O<jats:sub>2<\/jats:sub>in N<jats:sub>2<\/jats:sub>and 12% O<jats:sub>2<\/jats:sub>in N<jats:sub>2<\/jats:sub>, compared with the wild-type mice (C57BL\/6J) on the same genetic background as the NEP -\/- mice. Treatment of C57BL\/6J mice with thiorphan, a NEP inhibitor, resulted in a greater ventilatory response to 8% O<jats:sub>2<\/jats:sub>because of a significantly greater shortening of expiratory time. The results of these studies demonstrate that NEP plays an important role in modifying the expression of the ventilatory response to acute hypoxia.<\/jats:p>","DOI":"10.1152\/jappl.1999.87.4.1266","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T14:58:53Z","timestamp":1513954733000},"page":"1266-1271","source":"Crossref","is-referenced-by-count":19,"title":["Targeted deletion of the neutral endopeptidase gene alters ventilatory responses to acute hypoxia in mice"],"prefix":"10.1152","volume":"87","author":[{"given":"H.","family":"Grasemann","sequence":"first","affiliation":[{"name":"Combined Program in Pulmonary and Critical Care Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Lu","sequence":"additional","affiliation":[{"name":"Ina Sue Perlmutter Laboratory, Children\u2019s Hospital, Department of Medicine, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Jiao","sequence":"additional","affiliation":[{"name":"Combined Program in Pulmonary and Critical Care Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Boudreau","sequence":"additional","affiliation":[{"name":"Combined Program in Pulmonary and Critical Care Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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