{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:18:04Z","timestamp":1773296284565,"version":"3.50.1"},"reference-count":25,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2000,2,1]]},"abstract":"<jats:p>Carbonic anhydrase (CA) inhibition is associated with a lower plasma lactate concentration ([La<jats:sup>\u2212<\/jats:sup>]<jats:sub>pl<\/jats:sub>), but the mechanism for this association is not known. The effect of CA inhibition on muscle high-energy phosphates [ATP and phosphocreatine (PCr)], lactate ([La<jats:sup>\u2212<\/jats:sup>]<jats:sub>m<\/jats:sub>), and glycogen was examined in seven men [28 \u00b1 3 (SE) yr] during cycling exercise under control (Con) and acute CA inhibition with acetazolamide (Acz; 10 mg\/kg body wt iv). Subjects performed 6-min step transitions in work rate from 0 W to a work rate corresponding to \u223c50% of the difference between the O<jats:sub>2<\/jats:sub>uptake at the ventilatory threshold and peak O<jats:sub>2<\/jats:sub>uptake. Muscle biopsies were taken from the vastus lateralis at rest, at 30 min postinfusion, at end exercise (EE), and at 5 and 30 min postexercise. Arterialized venous blood was sampled from a dorsal hand vein and analyzed for [La<jats:sup>\u2212<\/jats:sup>]<jats:sub>pl<\/jats:sub>. ATP was unchanged from rest values; no difference between Con and Acz was observed. The fall in PCr from rest [72 \u00b1 3 and 73 \u00b1 3.6 (SE) mmol\/kg dry wt for Con and Acz, respectively] to EE (51 \u00b1 4 and 46 \u00b1 5 mmol\/kg dry wt for Con and Acz, respectively) was similar in Con and Acz. At EE, glycogen (mmol glucosyl units\/kg dry wt) decreased to similar values in Con and Acz (307 \u00b1 16 and 300 \u00b1 19, respectively). At EE, no difference was observed in [La<jats:sup>\u2212<\/jats:sup>]<jats:sub>m<\/jats:sub>between conditions (46 \u00b1 6 and 43 \u00b1 5 mmol\/kg dry wt for Con and Acz, respectively). EE [La<jats:sup>\u2212<\/jats:sup>]<jats:sub>pl<\/jats:sub>was higher during Con than during Acz (11.4 \u00b1 1.0 vs. 8.2 \u00b1 0.6 mmol\/l). The similar [La<jats:sup>\u2212<\/jats:sup>]<jats:sub>m<\/jats:sub>but lower [La<jats:sup>\u2212<\/jats:sup>]<jats:sub>pl<\/jats:sub>suggests that the uptake of La<jats:sup>\u2212<\/jats:sup>by other tissues is enhanced after CA inhibition.<\/jats:p>","DOI":"10.1152\/jappl.2000.88.2.722","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T16:34:12Z","timestamp":1513960452000},"page":"722-729","source":"Crossref","is-referenced-by-count":8,"title":["Muscle metabolism during heavy-intensity exercise after acute acetazolamide administration"],"prefix":"10.1152","volume":"88","author":[{"given":"Barry W.","family":"Scheuermann","sequence":"first","affiliation":[{"name":"Centre for Activity and Ageing, School of Kinesiology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Kowalchuk","sequence":"additional","affiliation":[{"name":"Centre for Activity and Ageing, School of Kinesiology, and"},{"name":"Department of Physiology, The University of Western Ontario, London, Ontario N6A 3K7; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald H.","family":"Paterson","sequence":"additional","affiliation":[{"name":"Centre for Activity and Ageing, School of Kinesiology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert W.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Centre for Activity and Ageing, School of Kinesiology, and"},{"name":"Department of Physiology, The University of Western Ontario, London, Ontario N6A 3K7; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard J.","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020960"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.77.4.1742"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1981.51.6.1662"},{"key":"B4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/00365516209075148","volume":"14","author":"Bergstr\u00f6m J.","year":"1962","journal-title":"Scand. 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