{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:16:59Z","timestamp":1773296219941,"version":"3.50.1"},"reference-count":41,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2000,7,1]]},"abstract":"<jats:p> The effects of acetazolamide (Acz)-induced carbonic anhydrase inhibition (CAI) on muscle intracellular thresholds (T) for intracellular pH (pH<jats:sub>i<\/jats:sub>) and inorganic phosphate-to-phosphate creatine ratio (P<jats:sub>i<\/jats:sub>\/PCr) and the plasma lactate (La<jats:sup>\u2212<\/jats:sup>) threshold were examined in nine adult male subjects performing forearm wrist flexion exercise to fatigue. Exercise consisted of raising and lowering (1-s contraction, 1-s relaxation) a cylinder whose volume increased at a rate of 200 ml\/min. The protocol was performed during control (Con) and after 45 min of CAI with Acz (10 mg\/kg body wt iv). T<jats:sub>pH<jats:sub>i<\/jats:sub> <\/jats:sub> and T<jats:sub>P<jats:sub>i<\/jats:sub> <\/jats:sub> <jats:sub>\/PCr<\/jats:sub>, determined using <jats:sup>31<\/jats:sup>P-labeled magnetic resonance spectroscopy (MRS), were similar in Acz (722 \u00b1 50 and 796 \u00b1 75 mW, respectively) and Con (855 \u00b1 211 and 835 \u00b1 235 mW, respectively). The pH<jats:sub>i<\/jats:sub> was similar at end-exercise (6.38 \u00b1 0.10 Acz and 6.43 \u00b1 0.22 Con), but pH<jats:sub>i<\/jats:sub>recovery was slowed in Acz. In a separate experiment, blood was sampled from a deep arm vein at the elbow for determination of plasma lactate concentration ([La<jats:sup>\u2212<\/jats:sup>]<jats:sub>pl<\/jats:sub>) and T<jats:sub>La<jats:sup>\u2212<\/jats:sup> <\/jats:sub>. [La<jats:sup>\u2212<\/jats:sup>]<jats:sub>pl<\/jats:sub> was lower ( P &lt; 0.05) in Acz than Con (3.7 \u00b1 1.7 vs. 5.0 \u00b1 1.7 mmol\/l) at end-exercise and in early recovery, but T<jats:sub>La<jats:sup>\u2212<\/jats:sup> <\/jats:sub> was higher (1,433 \u00b1 243 vs. 1,041 \u00b1 414 mW, respectively). These data suggest that the lower [La<jats:sup>\u2212<\/jats:sup>]<jats:sub>pl<\/jats:sub> seen with CAI was not due to a delayed onset or rate of muscle La<jats:sup>\u2212<\/jats:sup> accumulation but may be related to impaired La<jats:sup>\u2212<\/jats:sup> removal from muscle. <\/jats:p>","DOI":"10.1152\/jappl.2000.89.1.200","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T01:59:59Z","timestamp":1513994399000},"page":"200-209","source":"Crossref","is-referenced-by-count":21,"title":["Forearm muscle metabolism studied using <sup>31<\/sup>P-MRS during progressive exercise to fatigue after Acz administration"],"prefix":"10.1152","volume":"89","author":[{"given":"John M.","family":"Kowalchuk","sequence":"first","affiliation":[{"name":"The Centre for Activity and Ageing, School of Kinesiology and Departments of"},{"name":"Physiology and"},{"name":"Lawson Research Institute, St. Joseph's Health Centre, London, Ontario, Canada N6A 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