{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T19:50:17Z","timestamp":1787687417087,"version":"build-2784847793"},"reference-count":29,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2000,9,1]]},"abstract":"<jats:p>To examine the effect of exercise on heat shock protein (HSP) 72 mRNA expression in skeletal muscle, five healthy humans (20 \u00b1 1 yr; 64 \u00b1 3 kg; peak O<jats:sub>2<\/jats:sub>uptake of 2.55 \u00b1 0.2 l\/min) cycled until exhaustion at a workload corresponding to 63% peak O<jats:sub>2<\/jats:sub>uptake. Muscle was sampled from the vastus lateralis, and muscle temperature was measured at rest (R), 10 min of exercise (Min10), \u223c40 min before fatigue (F-40 = 144 \u00b1 7 min), and fatigue (F = 186 \u00b1 15 min). Muscle samples were analyzed for HSP72 mRNA expression, as well as glycogen and lactate concentration. Muscle temperature increased ( P &lt; 0.05) during the first 10 min of exercise but then remained constant for the duration of the exercise. Similarly, lactate concentration increased ( P &lt; 0.05) when Min10 was compared with R but decreased ( P &lt; 0.05) thereafter, such that concentrations at F-40 and F were not different from those at R. In contrast, muscle glycogen concentration fell progressively throughout exercise (486 \u00b1 74 vs. 25 \u00b1 7 mmol\/kg dry weight for R and F, respectively; P &lt; 0.05). HSP72 mRNA was detected at R but did not increase by Min10. However, HSP72 mRNA increased ( P &lt; 0.05) 2.2 \u00b1 0.5- and 2.6 \u00b1 0.9-fold, respectively, when F-40 and F were compared with R. These data demonstrate that HSP72 mRNA increases progressively during acute cycling, suggesting that processes that take place throughout concentric exercise are capable of initiating a stress response.<\/jats:p>","DOI":"10.1152\/jappl.2000.89.3.1055","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T01:16:21Z","timestamp":1513991781000},"page":"1055-1060","source":"Crossref","is-referenced-by-count":143,"title":["HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise"],"prefix":"10.1152","volume":"89","author":[{"given":"M. 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