{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T21:01:26Z","timestamp":1709586086271},"reference-count":20,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2000,10,1]]},"abstract":"<jats:p>Changes in plasma volume in vivo cause rapid changes in extracellular pH by altering the plasma bicarbonate concentration at a constant Pco<jats:sub>2<\/jats:sub>(Garella S, Chang BS, and Kahn SI. Kidney Int 8: 279, 1975). Few studies have examined the possibility that changes in cell volume produce comparable changes in intracellular pH (pH<jats:sub>i<\/jats:sub>). In the present study, alveolar macrophages were exposed to hyperosmotic medium in the absence or presence of the open-system buffers CO<jats:sub>2<\/jats:sub>-HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>, propionic acid-propionate, or NH<jats:sub>3<\/jats:sub>-NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup>. In the absence of open-system buffers, exposure to twice-normal osmolarity (2T) produced a slow cellular alkalinization [change in pH<jats:sub>i<\/jats:sub>(\u0394pH<jats:sub>i<\/jats:sub>) \u2248 0.38; exponential time constant (\u03c4) \u2248 120 s]. In the presence of 5% CO<jats:sub>2<\/jats:sub>, 2T caused a biphasic pH<jats:sub>i<\/jats:sub>response: a rapid increase (\u0394pH<jats:sub>i<\/jats:sub>\u2248 0.10, \u03c4 \u2248 15 s) followed by a slower pH<jats:sub>i<\/jats:sub>increase. Identical rapid pH<jats:sub>i<\/jats:sub>increases were produced by 2T in the presence of propionic acid (20 mM). Conversely, 2T caused a rapid pH<jats:sub>i<\/jats:sub>decrease (\u0394pH<jats:sub>i<\/jats:sub>\u2248 \u22120.21, \u03c4 \u2248 10 s) in the presence of NH<jats:sub>3<\/jats:sub>(20 mM). Thus osmotic cell shrinkage caused rapid pH<jats:sub>i<\/jats:sub>changes of opposite direction in the presence of a weak acid buffer (contraction alkalosis with CO<jats:sub>2<\/jats:sub>or propionic acid) vs. a weak base buffer (contraction acidosis with NH<jats:sub>3<\/jats:sub>). Graded \u0394pH<jats:sub>i<\/jats:sub>were produced by varying extracellular osmolarity in the presence of open-system buffers; osmolarity increases of as little as 5\u201310% produced significant \u0394pH<jats:sub>i<\/jats:sub>. The rapid pH<jats:sub>i<\/jats:sub>responses to 2T were insensitive to inhibitors of membrane H<jats:sup>+<\/jats:sup>transport (ethylisopropylamiloride and bafilomycin A<jats:sub>1<\/jats:sub>). The results are consistent with shrinkage-induced disequilibria in the total cellular buffer system (i.e., intrinsic buffers plus added weak acid-base buffer).<\/jats:p>","DOI":"10.1152\/jappl.2000.89.4.1543","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T00:57:01Z","timestamp":1513990621000},"page":"1543-1552","source":"Crossref","is-referenced-by-count":1,"title":["pH<sub>i<\/sub>responses to osmotic cell shrinkage in the presence of open-system buffers"],"prefix":"10.1152","volume":"89","author":[{"given":"Thomas A.","family":"Heming","sequence":"first","affiliation":[{"name":"Departments of Internal Medicine, and Physiology and Biophysics, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0876"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Boyarsky","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine, and Physiology and Biophysics, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0876"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Divina M.","family":"Tuazon","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine, and Physiology and Biophysics, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0876"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akhil","family":"Bidani","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine, and Physiology and Biophysics, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0876"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1994.266.6.L681"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1989.257.1.C65"},{"key":"B3","first-page":"252A","volume":"41","author":"Bidani A","year":"1993","journal-title":"Clin Res"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1995.269.1.L20"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.48.030186.002113"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.4.C1131"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.250.3.C423"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041220203"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1975.114"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869680"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.85.5.765"},{"key":"B13","doi-asserted-by":"crossref","first-page":"23823","DOI":"10.1016\/S0021-9258(18)35911-8","volume":"267","author":"Grinstein S","year":"1992","journal-title":"J Biol Chem"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.250.6.C920"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.57.4.609"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.80.5.1804"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.76.2.806"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.84.3.379"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1981.61.2.296"},{"key":"B20","first-page":"175","volume":"13","author":"Schlue WR","year":"1990","journal-title":"Renal Physiol Biochem"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1021\/bi00578a012"}],"container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/jappl.2000.89.4.1543","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T03:21:23Z","timestamp":1660188083000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/jappl.2000.89.4.1543"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10,1]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,10,1]]}},"alternative-id":["10.1152\/jappl.2000.89.4.1543"],"URL":"https:\/\/doi.org\/10.1152\/jappl.2000.89.4.1543","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10,1]]}}}