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In a high-PIP control group, K<jats:sub>fc<\/jats:sub>increased significantly after ventilation with 30 and 35 cmH<jats:sub>2<\/jats:sub>O PIP, but significant increases were prevented by treatment with 100 \u03bcM trifluoperazine, an inhibitor of Ca<jats:sup>2+<\/jats:sup>\/calmodulin, 500 nM ML-7, an inhibitor of myosin light chain kinase (MLCK), a combination of isoproterenol (20 \u03bcM) and rolipram (10 \u03bcM) to enhance intracellular cAMP levels, and a dose of KT-5720 (2 \u03bcM), which inhibits MLCK and protein kinase C. These studies suggest that the Ca<jats:sup>2+<\/jats:sup>\/calmodulin-MLCK pathway augments capillary fluid leak after a modest high-PIP injury and that this is attenuated by kinase inhibition and increased intracellular cAMP.<\/jats:p>","DOI":"10.1152\/jappl.2000.89.6.2241","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T21:47:46Z","timestamp":1513979266000},"page":"2241-2248","source":"Crossref","is-referenced-by-count":79,"title":["Inhibitors of myosin light chain kinase and phosphodiesterase reduce ventilator-induced lung injury"],"prefix":"10.1152","volume":"89","author":[{"given":"James C.","family":"Parker","sequence":"first","affiliation":[{"name":"Department of Physiology, University of South Alabama, Mobile, Alabama 36688"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"(With the Technical Assistance of Sherri L. 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