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Hyperoxia-induced retinal vasoobliteration in rat pups (80% O<jats:sub>2<\/jats:sub>exposure from postnatal days 5\u201314) was associated with increased TxB<jats:sub>2<\/jats:sub>generation and was significantly prevented by TxA<jats:sub>2<\/jats:sub>synthase inhibitor CGS-12970 (10 mg \u00b7 kg<jats:sup>\u22121<\/jats:sup>\u00b7 day<jats:sup>\u22121<\/jats:sup>) or TxA<jats:sub>2<\/jats:sub>-receptor antagonist CGS-22652 (10 mg \u00b7 kg<jats:sup>\u22121<\/jats:sup>\u00b7 day<jats:sup>\u22121<\/jats:sup>). TxA<jats:sub>2<\/jats:sub>mimetics U-46619 (EC<jats:sub>50<\/jats:sub>50 nM) and I-BOP (EC<jats:sub>50<\/jats:sub>5 nM) caused a time- and concentration-dependent cell death of neuroretinovascular endothelial cells from rats as well as newborn pigs but not of smooth muscle and astroglial cells; other prostanoids did not cause cell death. The peroxidation product 8-iso-PGF<jats:sub>2<\/jats:sub>, which is generated in OIR, stimulated TxA<jats:sub>2<\/jats:sub>formation by endothelial cells and triggered cell death; these effects were markedly diminished by CGS-12970. TxA<jats:sub>2<\/jats:sub>-dependent neuroretinovascular endothelial cell death was mostly by necrosis and to a lesser extent by apoptosis. The data identify an important role for TxA<jats:sub>2<\/jats:sub>in vasoobliteration of OIR and unveil a so far unknown function for TxA<jats:sub>2<\/jats:sub>in directly triggering neuroretinal microvascular endothelial cell death. These effects of TxA<jats:sub>2<\/jats:sub>might participate in other ischemic neurovascular injuries.<\/jats:p>","DOI":"10.1152\/jappl.2001.90.6.2279","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T21:38:33Z","timestamp":1513978713000},"page":"2279-2288","source":"Crossref","is-referenced-by-count":47,"title":["Role of thromboxane in retinal microvascular degeneration in oxygen-induced retinopathy"],"prefix":"10.1152","volume":"90","author":[{"given":"Martin H.","family":"Beauchamp","sequence":"first","affiliation":[{"name":"Departments of Pediatrics, Ophthalmology and Pharmacology, Research Center, H\u00f4pital Sainte-Justine, Montreal, Quebec H3T 1C5; Departments of"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Katherine","family":"Martinez-Bermudez","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics, Ophthalmology and Pharmacology, Research Center, H\u00f4pital Sainte-Justine, Montreal, Quebec 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