{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:30:37Z","timestamp":1792654237235,"version":"4.5.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p> The purpose of this study was to determine whether there are alterations in the dihydropyridine and\/or ryanodine receptors that might explain the excitation-contraction uncoupling associated with eccentric contraction-induced skeletal muscle injury. The left anterior crural muscles (i.e., tibialis anterior, extensor digitorum longus, and extensor hallucis longus) of mice were injured in vivo by 150 eccentric contractions. Peak isometric tetanic torque of the anterior crural muscles was reduced \u223c45% immediately and 3 days after the eccentric contractions. Partial restoration of peak isometric tetanic and subtetanic forces of injured extensor digitorum longus muscles by 10 mM caffeine indicated the presence of excitation-contraction uncoupling. Scatchard analysis of [<jats:sup>3<\/jats:sup>H]ryanodine binding indicated that the number of ryanodine receptor binding sites was not altered immediately postinjury but decreased 16% 3 days later. Dihydropyridine receptor binding sites increased \u223c20% immediately after and were elevated to the same extent 3 days after the injury protocol. Muscle injury did not alter the sensitivity of either receptor. These data suggest that a loss or altered sensitivity of the dihydropyridine and ryanodine receptors does not contribute to the excitation-contraction uncoupling immediately after contraction-induced muscle injury. We also concluded that the loss in ryanodine receptors 3 days after injury is not the primary cause of excitation-contraction uncoupling at that time. <\/jats:p>","DOI":"10.1152\/japplphysiol.00084.2003","type":"journal-article","created":{"date-parts":[[2004,4,8]],"date-time":"2004-04-08T20:57:57Z","timestamp":1081457877000},"page":"1619-1625","source":"Crossref","is-referenced-by-count":42,"title":["Dihydropyridine and ryanodine receptor binding after eccentric contractions in mouse skeletal muscle"],"prefix":"10.1152","volume":"96","author":[{"given":"Christopher P.","family":"Ingalls","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon L.","family":"Warren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Zheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. B.","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.2.C462"},{"key":"REF2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020943"},{"key":"REF3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.649bj.x"},{"key":"REF4","doi-asserted-by":"publisher","DOI":"10.1038\/320168a0"},{"key":"REF5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.6.C1587"},{"key":"REF6","doi-asserted-by":"publisher","DOI":"10.1139\/h99-020"},{"key":"REF7","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900063"},{"key":"REF8","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1977.sp012072"},{"key":"REF9","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.87.2.471"},{"key":"REF10","doi-asserted-by":"publisher","DOI":"10.1007\/BF00375074"},{"key":"REF11","unstructured":"Firestone LL, Alifimoff JK, and Miller KW. Does general anestheticinduced desensitization of the Torpedo acetylcholine receptor correlate with lipid disordering. Mol Pharmacol 46: 508-515, 1994."},{"key":"REF12","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199505000-00023"},{"key":"REF13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.13961"},{"key":"REF14","unstructured":"Hamilton SL, Yatani A, Brush K, Schwartz A, and Brown AM. A comparison between the binding and electrophysiological effects of dihydropyridine on cardiac membranes. Mol Pharmacol 31: 221-231, 1987."},{"key":"REF15","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005496708505"},{"key":"REF16","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019629"},{"key":"REF17","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005368831198"},{"key":"REF18","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.80.1.332"},{"key":"REF19","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.85.1.58"},{"key":"REF20","doi-asserted-by":"crossref","unstructured":"Ingalls CP, Wenke JC, Nofal T, Hinz S, Arnold E, and Armstrong RB. Mechanisms of strength adaptation after repeated bouts of exercise-induced muscle injury (Abstract). Med Sci Sports Exerc 31: S41, 2001.","DOI":"10.1097\/00005768-200105001-00233"},{"key":"REF21","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.77.4.1926"},{"key":"REF22","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1995.79.4.1260"},{"key":"REF23","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1985.59.1.119"},{"key":"REF24","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7712"},{"key":"REF25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.43.25837"},{"key":"REF26","doi-asserted-by":"crossref","unstructured":"Reggiani C, Brocks L, Wirtz P, Loermans H, and Kronnie G. Myosin isoforms in hindlimb muscles of normal and dystrophic (ReJ129 dy\/dy) mice. Muscle Nerve 14: 199-208, 1992.","DOI":"10.1002\/mus.880150212"},{"key":"REF27","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1991.71.3.849"},{"key":"REF28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114504"},{"key":"REF29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.5.C1475"},{"key":"REF30","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79446-8"},{"key":"REF31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0571a.x"},{"key":"REF32","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.77.3.1421"},{"key":"REF33","doi-asserted-by":"publisher","DOI":"10.1097\/00003677-200104000-00008"},{"key":"REF34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.609ac.x"},{"key":"REF35","doi-asserted-by":"publisher","DOI":"10.1007\/BF00319121"},{"key":"REF36","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019783"},{"key":"REF37","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.81.6.2555"},{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1007\/s004210000275"},{"key":"REF39","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013839"},{"key":"REF40","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0402fje"},{"key":"REF41","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.74.2.271"},{"key":"REF42","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.9.908"}],"container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/japplphysiol.00084.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:03:14Z","timestamp":1567958594000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/japplphysiol.00084.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["10.1152\/japplphysiol.00084.2003"],"URL":"https:\/\/doi.org\/10.1152\/japplphysiol.00084.2003","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}