{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:23:38Z","timestamp":1791156218633,"version":"4.1.0"},"reference-count":34,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:p> The goal of this study was to use the model of spinal cord isolation (SI), which blocks nearly all neuromuscular activity while leaving the motoneuron muscle-fiber connections intact, to characterize the cellular processes linked to marked muscle atrophy. Rats randomly assigned to normal control and SI groups were studied at 0, 2, 4, 8, and 15 days after SI surgery. The slow soleus muscle atrophied by \u223c50%, with the greatest degree of loss occurring during the first 8 days. Throughout the SI duration, muscle protein concentration was maintained at the control level, whereas myofibrillar protein concentration steadily decreased between 4 and 15 days of SI, and this was associated with a 50% decrease in myosin heavy chain (MHC) normalized to total protein. Actin relative to the total protein was maintained at the control level. Marked reductions occurred in total RNA and DNA content and in total MHC and actin mRNA expressed relative to 18S ribosomal RNA. These findings suggest that two key factors contributing to the muscle atrophy in the SI model are 1) a reduction in ribosomal RNA that is consistent with a reduction in protein translational capacity, and 2) insufficient mRNA substrate for translating key sarcomeric proteins comprising the myofibril fraction, such as MHC and actin. In addition, the marked selective depletion of MHC protein in the muscles of SI rats suggests that this protein is more vulnerable to inactivity than actin protein. This selective MHC loss could be a major contributor for the previously reported loss in the functional integrity of SI muscles. Collectively, these data are consistent with the involvement of pretranslational and translational processes in muscle atrophy due to SI. <\/jats:p>","DOI":"10.1152\/japplphysiol.00317.2003","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T15:46:02Z","timestamp":1425397562000},"page":"781-790","source":"Crossref","is-referenced-by-count":71,"title":["Atrophy responses to muscle inactivity. I. Cellular markers of protein  deficits"],"prefix":"10.1152","volume":"95","author":[{"given":"F.","family":"Haddad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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A reagent for the single-step simultaneous  isolation of RNA, DNA and proteins from cell and tissue samples.  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Weightlessness simulations for cardiovascular and muscle  systems: validity of rat models. J Gravit Physiol 4: 49-59,  1997."},{"key":"REF25","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880100509"},{"key":"REF26","doi-asserted-by":"publisher","DOI":"10.1249\/00003677-199600240-00015"},{"key":"REF27","unstructured":"Roy RR, Hodgson  JA, Lauretz SD, Pierotti DJ, Gayek RJ, and Edgerton VR. Chronic spinal  cord-injured cats: surgical procedures and management. Lab Anim  Sci 42: 335-343,  1992."},{"key":"REF28","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(200005)23:5<735::AID-MUS11>3.0.CO;2-T"},{"key":"REF29","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10219"},{"key":"REF30","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(71)90033-8"},{"key":"REF31","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(200005)23:5<661::AID-MUS3>3.0.CO;2-J"},{"key":"REF32","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.75.5.2337"},{"key":"REF33","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1990.68.1.1"},{"key":"REF34","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900670107"}],"container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/japplphysiol.00317.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:38:49Z","timestamp":1567960729000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/japplphysiol.00317.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1152\/japplphysiol.00317.2003"],"URL":"https:\/\/doi.org\/10.1152\/japplphysiol.00317.2003","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8]]}}}