{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T10:42:49Z","timestamp":1772793769092,"version":"3.50.1"},"reference-count":59,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p> This study was designed to determine whether chronic heart failure (CHF) results in changes in Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase properties in heart and skeletal muscles of different fiber-type composition. Adult rats were randomly assigned to a control (Con; n = 8) or CHF ( n = 8) group. CHF was induced by ligation of the left main coronary artery. Examination of Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase activity (means \u00b1 SE) 12 wk after the ligation measured, using the 3- O-methylfluorescein phosphatase assay (3- O-MFPase), indicated higher ( P &lt; 0.05) levels in soleus (Sol) (250 \u00b1 13 vs. 179 \u00b1 18 nmol\u00b7mg protein<jats:sup>\u22121<\/jats:sup>\u00b7h<jats:sup>\u22121<\/jats:sup>) and lower ( P &lt; 0.05) levels in diaphragm (Dia) (200 \u00b1 12 vs. 272 \u00b1 27 nmol\u00b7mg protein<jats:sup>\u22121<\/jats:sup>\u00b7h<jats:sup>\u22121<\/jats:sup>) and left ventricle (LV) (760 \u00b1 62 vs. 992 \u00b1 16 nmol\u00b7mg protein<jats:sup>\u22121<\/jats:sup>\u00b7h<jats:sup>\u22121<\/jats:sup>) in CHF compared with Con, respectively. Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase protein content, measured by the [<jats:sup>3<\/jats:sup>H]ouabain binding technique, was higher ( P &lt; 0.05) in white gastrocnemius (WG) (166 \u00b1 12 vs. 135 \u00b1 7.6 pmol\/g wet wt) and lower ( P &lt; 0.05) in Sol (193 \u00b1 20 vs. 260 \u00b1 8.6 pmol\/g wet wt) and LV (159 \u00b1 10 vs. 221 \u00b1 10 pmol\/g wet wt) in CHF compared with Con, respectively. Isoform content in CHF, measured by Western blot techniques, showed both increases (WG; P &lt; 0.05) and decreases (Sol; P &lt; 0.05) in \u03b1<jats:sub>1<\/jats:sub>. For \u03b1<jats:sub>2<\/jats:sub>, only increases [red gastrocnemius (RG), Sol, and Dia; P &lt; 0.05] occurred. The \u03b2<jats:sub>2<\/jats:sub>-isoform was decreased (LV, Sol, RG, and WG; P &lt; 0.05) in CHF, whereas the \u03b2<jats:sub>1<\/jats:sub> was both increased (WG and Dia; P &lt; 0.05) and decreased (Sol and LV; P &lt; 0.05). For \u03b2<jats:sub>3<\/jats:sub>, decreases ( P &lt; 0.05) in RG were observed in CHF, whereas no differences were found in Sol and WG between CHF and Con. It is concluded that CHF results in alterations in Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase that are muscle specific and property specific. Although decreases in Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase content would appear to explain the lower 3- O-MFPase in the LV, such does not appear to be the case in skeletal muscles where a dissociation between these properties was observed. <\/jats:p>","DOI":"10.1152\/japplphysiol.00343.2004","type":"journal-article","created":{"date-parts":[[2005,4,7]],"date-time":"2005-04-07T20:34:18Z","timestamp":1112906058000},"page":"656-664","source":"Crossref","is-referenced-by-count":12,"title":["Na<sup>+<\/sup>-K<sup>+<\/sup>-ATPase properties in rat heart and skeletal muscle 3 mo after coronary artery ligation"],"prefix":"10.1152","volume":"99","author":[{"given":"D. J.","family":"Barr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. J.","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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