{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:37:28Z","timestamp":1791398248371,"version":"4.3.3"},"reference-count":56,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/501100000024","name":"Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de recherche en sant\u00e9 du Canada)","doi-asserted-by":"publisher","award":["MOP-102715"],"award-info":[{"award-number":["MOP-102715"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:p>We tested the hypothesis that membrane progesterone receptors (mPR) contribute to respiratory control in adult male and female mice. Mice were implanted with osmotic minipumps for continuous infusion of small interfering RNA (siRNA) directed against mPR\u03b1, mPR\u03b2, or a control solution in the fourth ventricle (to target brain stem respiratory areas) for 14 days. We then performed respiratory and metabolic recordings by whole body plethysmography at rest and in response to hypoxia (12% O<jats:sub>2<\/jats:sub>) or hypercapnia (5% CO<jats:sub>2<\/jats:sub>, 5 min each). For each treatment, we have verified with immunohistochemistry that the staining intensity of mPR\u03b1 or mPR\u03b2 in the brain stem is decreased. At rest, the siRNA against mPR\u03b1 and mPR\u03b2 increased respiratory frequency in males only. The siRNA against mPR\u03b2 almost tripled the frequency of apneas in male and in female mice, while the siRNA against mPR\u03b1 had no effect. Regarding respiratory chemoreflex, the siRNA against mPR\u03b2 suppressed the response to hypoxia in male and female mice and reduced by \u223c50% the response to hypercapnia, while the siRNA against mPR\u03b1 had more limited effects. Interestingly, control females had higher ventilatory response to hypoxia and hypercapnia than males, and these sex-specific effects were suppressed by the siRNA against mPR\u03b2, whereas they were still present after treatment with the siRNA against mPR\u03b1. We conclude that mPR\u03b2 reduces apnea frequency in male and female mice and establishes sex-specific ventilatory chemoreflex.<\/jats:p>","DOI":"10.1152\/japplphysiol.00397.2016","type":"journal-article","created":{"date-parts":[[2016,7,29]],"date-time":"2016-07-29T03:23:49Z","timestamp":1469762629000},"page":"781-791","source":"Crossref","is-referenced-by-count":34,"title":["Membrane progesterone receptor-\u03b2, but not -\u03b1, in dorsal brain stem establishes sex-specific chemoreflex responses and reduces apnea frequency in adult mice"],"prefix":"10.1152","volume":"121","author":[{"given":"Ryma","family":"Boukari","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche en P\u00e9rinatologie, Centre Hospitalier Universitaire de Qu\u00e9bec, H\u00f4pital Saint-Fran\u00e7ois d'Assise, D\u00e9partement de P\u00e9diatrie, Universit\u00e9 Laval, Qu\u00e9bec, 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