{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:28:22Z","timestamp":1791293302045,"version":"4.1.0"},"reference-count":58,"publisher":"American Physiological Society","issue":"6","funder":[{"name":"Rett Syndrome Research Trust","award":["None"],"award-info":[{"award-number":["None"]}]},{"DOI":"10.13039\/100001819","name":"International Rett Syndrome Foundation (IRSF)","doi-asserted-by":"publisher","award":["None"],"award-info":[{"award-number":["None"]}],"id":[{"id":"10.13039\/100001819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2015,9,15]]},"abstract":"<jats:p>Mice that are deficient in the transcription factor methyl-CpG-binding protein 2 (MeCP2) have a depressed hypercapnic ventilatory response (HCVR). The expression of MeCP2 can be selectively removed from astrocytes or neurons, thus offering a tool to dissect the role of this transcription factor in astrocytes from that in neurons. Studies were carried out in the progeny of mice that were a cross between those harboring a tamoxifen (TAM)-inducible Cre recombinase transgene driven by the human astrocytic glial fibrillary acidic protein (hGFAP) promoter, or Cre recombinase under control of the synapsin promoter, with mice containing a Cre-excisable exon III in the Mecp2 gene. The TAM-conditional excision of the Mecp2 exon allowed the respiratory CO<jats:sub>2<\/jats:sub>response to be studied in the same animals before and after selective depletion of MeCP2 in astrocytes. Immunohistochemistry showed that following TAM treatment only \u223c20% of GFAP-labeled cells in the retrotrapazoid nucleus and in the raph\u00e9 magnus were positive for MeCP2. The slope of the relative increase in minute ventilation as a function of 1, 3, and 5% inspired CO<jats:sub>2<\/jats:sub>was depressed in mice with depleted astrocyte MeCP2 compared with wild-type littermates. In contrast, selective depletion of MeCP2 in neurons did not significantly affect slope. While neurons which constitute the respiratory network ultimately determine the ventilatory response to CO<jats:sub>2<\/jats:sub>, this study demonstrates that loss of MeCP2 in astrocytes alone is sufficient to result in a dramatic attenuation of the HCVR. We propose that the glial contribution to HCVR is under the control of the MeCP2 gene.<\/jats:p>","DOI":"10.1152\/japplphysiol.00411.2015","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T02:40:38Z","timestamp":1437705638000},"page":"670-676","source":"Crossref","is-referenced-by-count":33,"title":["Conditional depletion of methyl-CpG-binding protein 2 in astrocytes depresses the hypercapnic ventilatory response in mice"],"prefix":"10.1152","volume":"119","author":[{"given":"Saurabh K.","family":"Garg","sequence":"first","affiliation":[{"name":"Vollum Institute,"},{"name":"Howard Hughes Medical Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel T.","family":"Lioy","sequence":"additional","affiliation":[{"name":"Vollum Institute,"},{"name":"Howard Hughes Medical Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon 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