{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:35:03Z","timestamp":1758926103175},"reference-count":50,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p>Mechanical properties of spontaneously contracting isolated nonpregnant human myometrium (NPHM) were investigated throughout the whole continuum of load from zero load up to isometry. This made it possible to assess the three-dimensional tension-velocity-length (T-V-L) relationship characterizing the level of contractility and to determine crossbridge (CB) kinetics of myosin molecular motors. Seventy-seven muscle strips were obtained from hysterectomy in 42 nonpregnant patients. Contraction and relaxation parameters were measured during spontaneous mechanical activity. The isotonic tension-peak velocity (T-V) relationship was hyperbolic in 30 cases and nonhyperbolic in 47 cases. When the T-V relationship was hyperbolic, the Huxley formalism could be used to calculate CB kinetics and CB unitary force. At the whole muscle level and for a given isotonic load level, part of the V-L phase plane showed a common pathway, so that a given instantaneous length corresponded to only one possible instantaneous velocity, independent of time and initial length. At the molecular level, rate constants for CB attachment and detachment were dramatically low, \u223c100 times lower than those of striated muscles, and \u223c5 to 10 times lower than those of other smooth muscles. The CB unitary force was \u223c1.4 \u00b1 0.1 pN. NPHM shared similar basic contractile properties with striated muscles, reflected in the three-dimensional T-V-L relationship characterizing the contractile level. Low CB attachment and detachment rate constants made it possible to generate normal CB unitary force and normal muscle tension in NPHM, even though it contracted extremely slowly compared with other muscles.<\/jats:p>","DOI":"10.1152\/japplphysiol.00414.2011","type":"journal-article","created":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T03:02:35Z","timestamp":1311303755000},"page":"1096-1105","source":"Crossref","is-referenced-by-count":3,"title":["Mechanics and energetics of myosin molecular motors from nonpregnant human myometrium"],"prefix":"10.1152","volume":"111","author":[{"given":"Edouard R.","family":"Lecarpentier","sequence":"first","affiliation":[{"name":"Service de Gyn\u00e9cologie-Obst\u00e9trique,"},{"name":"Centre de Recherche Clinique, Centre Hospitalier R\u00e9gional de Meaux, Meaux, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor A.","family":"Claes","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical 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