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Exercise training improves muscle insulin sensitivity, but its effects on intestinal metabolism are not known. We studied the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on intestinal glucose and free fatty acid uptake from circulation in humans. Twenty-eight healthy, middle-aged, sedentary men were randomized for 2 wk of HIIT or MICT. Intestinal insulin-stimulated glucose uptake and fasting free fatty acid uptake from circulation were measured using positron emission tomography and [<jats:sup>18<\/jats:sup>F]FDG and [<jats:sup>18<\/jats:sup>F]FTHA. In addition, effects of HIIT and MICT on intestinal GLUT2 and CD36 protein expression were studied in rats. Training improved aerobic capacity ( P = 0.001) and whole body insulin sensitivity ( P = 0.04), but not differently between HIIT and MICT. Insulin-stimulated glucose uptake increased only after the MICT in the colon (HIIT\u2009=\u20090%; MICT\u2009=\u200937%) ( P = 0.02 for time \u00d7 training) and tended to increase in the jejunum (HIIT\u2009=\u2009\u22124%; MICT\u2009=\u200913%) ( P = 0.08 for time \u00d7 training). Fasting free fatty acid uptake decreased in the duodenum in both groups (HIIT\u2009=\u2009\u22126%; MICT\u2009=\u2009\u221248%) ( P = 0.001 time) and tended to decrease in the colon in the MICT group (HIIT\u2009=\u20090%; MICT\u2009=\u2009\u221238%) ( P = 0.08 for time \u00d7 training). In rats, both training groups had higher GLUT2 and CD36 expression compared with control animals. This study shows that already 2 wk of MICT enhances insulin-stimulated glucose uptake, while both training modes reduce fasting free fatty acid uptake in the intestine in healthy, middle-aged men, providing an additional mechanism by which exercise training can improve whole body metabolism.<\/jats:p><jats:p>NEW &amp; NOTEWORTHY This is the first study where the effects of exercise training on the intestinal substrate uptake have been investigated using the most advanced techniques available. We also show the importance of exercise intensity in inducing these changes.<\/jats:p>","DOI":"10.1152\/japplphysiol.00431.2016","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T01:10:41Z","timestamp":1486689041000},"page":"1188-1197","source":"Crossref","is-referenced-by-count":22,"title":["Two weeks of moderate-intensity continuous training, but not high-intensity interval training, increases insulin-stimulated intestinal glucose uptake"],"prefix":"10.1152","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-0120","authenticated-orcid":false,"given":"Kumail K.","family":"Motiani","sequence":"first","affiliation":[{"name":"Turku PET Centre, University of Turku, Turku, Finland;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna M.","family":"Savolainen","sequence":"additional","affiliation":[{"name":"Turku PET Centre, University of Turku, Turku, 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