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Reconstructed image data were translated into local mineral densities by using a calibrated linear relationship between linear absorption coefficients and concentrations of homogeneous K<jats:sub>2<\/jats:sub>HPO<jats:sub>4<\/jats:sub> solution. Pure bone three-dimensional images, produced by simple thresholding at a bone mineral density of 0.82 g\/cm<jats:sup>3<\/jats:sup>, were analyzed for macro- and microscopic structural properties. In neurectomized hindlimbs, cortical canal network rarefaction as well as bone atrophy were found. The former was characterized by 30% smaller porosity, 11% smaller canal density in transverse section, and 38% smaller canal connectivity density than those in contralateral bone. On the other hand, no difference was found in bone mineral density between neurectomized and intact hindlimbs (1.37 vs. 1.36 g\/cm<jats:sup>3<\/jats:sup>). In conclusion, SR\u03bcCT is a promising method for the three-dimensional analysis of cortical microstructure and the degree of mineralization in small animals. <\/jats:p>","DOI":"10.1152\/japplphysiol.00495.2005","type":"journal-article","created":{"date-parts":[[2005,9,3]],"date-time":"2005-09-03T00:45:22Z","timestamp":1125708322000},"page":"274-280","source":"Crossref","is-referenced-by-count":41,"title":["Monochromatic synchrotron radiation \u03bcCT reveals disuse-mediated canal network rarefaction in cortical bone of growing rat tibiae"],"prefix":"10.1152","volume":"100","author":[{"given":"Takeshi","family":"Matsumoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Yoshino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahisa","family":"Asano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Uesugi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Todoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Bonse U and Busch F. 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