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In this study, one group of female young (10\u201312 wk) and old (22\u201324 mo) C57BL\/6 mice was exposed to a single bout of intense exercise to exhaustion and immediately infected with lymphocytic choriomeningitis virus (LCMV). Eight days later, at the peak of expansion phase of T-cell response, we used tetramers of MHC class I molecules containing viral peptides to directly visualize antigen-specific CD8 T cells and a sensitive functional assay measuring interferon-\u03b3 production at the single-cell level to quantitate the CD8 and CD4 T-cell response. To evaluate the impact of intense exercise during both the initiation and evolution of the expansion phase of the T-cell response, a second group of young and old mice continued their daily bouts of intense exercise to exhaustion over the next 8 days. Our data show that, in young mice, LCMV infection following exhaustive exercise leads to suppression of LCMV-specific CD8 and CD4 T-cell responses, and this suppression effect occurs at the initiation of the expansion phase of viral-specific T cells. However, in old mice, unlike young mice, exhaustive exercise does not cause suppression of LCMV-specific T-cell responses.<\/jats:p>","DOI":"10.1152\/japplphysiol.00510.2004","type":"journal-article","created":{"date-parts":[[2004,9,25]],"date-time":"2004-09-25T00:23:51Z","timestamp":1096071831000},"page":"663-671","source":"Crossref","is-referenced-by-count":10,"title":["Suppression of viral specific primary T-cell response following intense physical exercise in young but not old mice"],"prefix":"10.1152","volume":"98","author":[{"given":"Zoher F.","family":"Kapasi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"McRae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafi","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5258.54"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.2.521"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Asano MSand Ahmed R.Immune conflicts in lymphocytic choriomeningitis virus.Springer Semin Immunopathol17: 247\u2013259, 1995.","DOI":"10.1007\/BF00196168"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.86.6.1823"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1007\/BF01310513"},{"key":"R6","unstructured":"Berk LS, Nieman DC, Youngberg WS, Arabatzis K, Simpson-Westerberg M, Lee JW, Tan SA, and Eby WC.The effect of long endurance running on natural killer cells in marathoners.Med Sci Sports Exerc22: 207\u2013212, 1990."},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00918251"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80469-0"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.3.C626"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Ernst DN, Hobbs MV, Torbett BE, Glasebrook AL, Rehse MA, Bottomly K, Hayakawa K, Hardy RR, and Weigle WO.Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+T cells from young and aged mice.J Immunol145: 1295\u20131302, 1990.","DOI":"10.4049\/jimmunol.145.5.1295"},{"key":"R11","doi-asserted-by":"crossref","unstructured":"Ernst DN, Weigle WO, Noonan DJ, McQuitty DN, and Hobbs MV.The age-associated increase in IFN-gamma synthesis by mouse CD8+T cells correlates with shifts in the frequencies of cell subsets defined by membrane CD44, CD45RB, 3G11, and MEL-14 expression.J Immunol151: 575\u2013587, 1993.","DOI":"10.4049\/jimmunol.151.2.575"},{"key":"R12","unstructured":"Felsenthal G, Ference TS, and Young MA.Aging of organ systems. 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