{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T18:27:58Z","timestamp":1788632878862,"version":"build-2803163510"},"reference-count":33,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2012,1,1]]},"abstract":"<jats:p>Utilizing micro-X-ray CT (\u03bcCT) imaging, we sought to generate an atlas of in vivo and intact\/ex vivo lungs from normal murine strains. In vivo imaging allows visualization of parenchymal density and small airways (15\u201328 \u03bcm\/voxel). Ex vivo imaging of the intact lung via \u03bcCT allows for improved understanding of the three-dimensional lung architecture at the alveolar level with voxel dimensions of 1\u20132 \u03bcm. \u03bcCT requires that air spaces remain air-filled to detect alveolar architecture while in vivo structural geometry of the lungs is maintained. To achieve these requirements, a fixation and imaging methodology that permits nondestructive whole lung ex vivo \u03bcCT imaging has been implemented and tested. After in vivo imaging, lungs from supine anesthetized C57Bl\/6 mice, at 15, 20, and 25 cmH<jats:sub>2<\/jats:sub>O airway pressure, were fixed in situ via vascular perfusion using a two-stage flushing system while held at 20 cmH<jats:sub>2<\/jats:sub>O airway pressure. Extracted fixed lungs were air-dried. Whole lung volume was acquired at 1, 7, 21, and &gt;70 days after the lungs were dried and served as validation for fixation stability. No significant shrinkage was observed: +8.95% change from in vivo to fixed lung ( P = 0.12), \u22121.47% change from day 1 to day 7 ( P = 0.07), \u22122.51% change from day 1 to day 21 ( P = 0.05), and \u22124.90% change from day 1 to day 70 and thereafter ( P = 0.04). \u03bcCT evaluation showed well-fixed alveoli and capillary beds correlating with histological analysis. A fixation and imaging method has been established for \u03bcCT imaging of the murine lung that allows for ex vivo morphometric analysis, representative of the in vivo lung.<\/jats:p>","DOI":"10.1152\/japplphysiol.00550.2011","type":"journal-article","created":{"date-parts":[[2011,8,5]],"date-time":"2011-08-05T04:23:24Z","timestamp":1312518204000},"page":"159-166","source":"Crossref","is-referenced-by-count":52,"title":["Optimized murine lung preparation for detailed structural evaluation via micro-computed tomography"],"prefix":"10.1152","volume":"112","author":[{"given":"Drago\u015f M.","family":"Vasilescu","sequence":"first","affiliation":[{"name":"Department of Radiology, University of Iowa, Iowa City, Iowa;"},{"name":"Department of Diagnostic Radiology, Philipps University, Marburg;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Knudsen","sequence":"additional","affiliation":[{"name":"Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Ochs","sequence":"additional","affiliation":[{"name":"Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewald R.","family":"Weibel","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Bern University, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric A.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Department of Radiology, University of Iowa, Iowa City, Iowa;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1982.53.2.528"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1982.53.4.879"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1080\/01902140490242849"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11791"},{"key":"B6","first-page":"159","volume":"26","author":"Chang CH","year":"2006","journal-title":"Anticancer Res"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1097\/01.rli.0000160070.67270.05"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0414"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00010104"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1979.47.5.990"},{"key":"B11","first-page":"283","author":"Guo J","journal-title":"Proc 1st Int Workshop Pulm Image Anal 2008"},{"key":"B12","unstructured":"Heitzman ER.The Lung, Radiologic-Pathologic Correlations. 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