{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T15:35:47Z","timestamp":1792424147863,"version":"4.5.0"},"reference-count":32,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:p>Eugenol, a methoxyphenol component of clove oil, suppresses cyclooxygenase-2 expression, while eugenol dimers prevent nuclear factor-\u03baB (NF-\u03baB) activation and inflammatory cytokine expression in lipopolysaccharide-stimulated macrophages. Our aim was to examine the in vivo anti-inflammatory effects of eugenol. BALB\/c mice were divided into four groups. Mice received saline [0.05 ml intratracheally (it), control (Ctrl) and eugenol (Eug) groups] or Escherichia coli LPS (10 \u03bcg it, LPS and LPSEug groups). After 6 h, mice received saline (0.2 ml ip, Ctrl and LPS groups) or eugenol (160 mg\/kg ip, Eug and LPSEug groups). Twenty-four hours after LPS injection, pulmonary resistive (\u0394P1) and viscoelastic (\u0394P2) pressures, static elastance (E<jats:sub>st<\/jats:sub>), and viscoelastic component of elastance (\u0394E) were measured. Lungs were prepared for histology. In parallel mice, bronchoalveolar lavage fluid was collected 24 h after LPS injection. TNF-\u03b1 was determined by ELISA. Lung tissue expression of NF-\u03baB was determined by EMSA. \u0394P1, \u0394P2, E<jats:sub>st<\/jats:sub>, and \u0394E were significantly higher in the LPS group than in the other groups. LPS mice also showed significantly more alveolar collapse, collagen fibers, and neutrophil influx and higher TNF-\u03b1 levels and NF-\u03baB expression than the other groups. Eugenol treatment reduced LPS-induced lung inflammation, improving lung function. Our results suggest that eugenol exhibits in vivo anti-inflammatory action in LPS-induced lung injury.<\/jats:p>","DOI":"10.1152\/japplphysiol.00560.2009","type":"journal-article","created":{"date-parts":[[2010,1,15]],"date-time":"2010-01-15T01:58:14Z","timestamp":1263520694000},"page":"845-851","source":"Crossref","is-referenced-by-count":95,"title":["In vivo anti-inflammatory action of eugenol on lipopolysaccharide-induced lung injury"],"prefix":"10.1152","volume":"108","author":[{"given":"Clarissa B.","family":"Magalh\u00e3es","sequence":"first","affiliation":[{"name":"Laboratory of Respiration Physiology, Carlos Chagas Filho Biophysics Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas R.","family":"Riva","sequence":"additional","affiliation":[{"name":"Laboratory of Respiration Physiology, Carlos Chagas Filho Biophysics Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo J.","family":"DePaula","sequence":"additional","affiliation":[{"name":"Laboratory of Respiration Physiology, Carlos Chagas Filho Biophysics Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Brando-Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Immunopharmacology, Medical Biochemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro; 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