{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T08:36:28Z","timestamp":1789806988181,"version":"4.0.1"},"reference-count":56,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2002,10,1]]},"abstract":"<jats:p>It has been hypothesized that O<jats:sub>2<\/jats:sub>sensing in type I cells of the carotid body and erythropoietin (EPO)-producing cells of the kidney involves protein components identical to the NADPH oxidase system responsible for the respiratory burst of phagocytes. In the present study, we evaluated O<jats:sub>2<\/jats:sub>sensing in mice with null mutant genotypes for two components of the phagocytic oxidase. Whole body plethysmography was used to study unanesthetized, unrestrained mice. When exposed to an acute hypoxic stimulus, gp91<jats:sup>phox<\/jats:sup>-null mutant and wild-type mice increased their minute ventilation by similar amounts. In contrast, p47<jats:sup>phox<\/jats:sup>-null mutant mice demonstrated increases in minute ventilation in response to hypoxia that exceeded that of their wild-type counterparts: 98.0 \u00b1 18.0 vs. 20.0 \u00b1 13.0% ( n = 11, P = 0.003). In vitro recordings of carotid sinus nerve (CSN) activity demonstrated that resting (basal) neural activity was marginally elevated in p47<jats:sup>phox<\/jats:sup>-null mutant mice. With hypoxic challenge, mean CSN discharge was 1.5-fold greater in p47<jats:sup>phox<\/jats:sup>-null mutant than in wild-type mice: 109.61 \u00b1 13.29 vs. 72.54 \u00b1 7.65 impulses\/s ( n = 8 and 7, respectively, P = 0.026). Consequently, the hypoxia-evoked CSN discharge (stimulus-basal) was \u223c58% larger in p47<jats:sup>phox<\/jats:sup>-null mutant mice. Quantities of EPO mRNA in kidney were similar in gp91<jats:sup>phox<\/jats:sup>- and p47<jats:sup>phox<\/jats:sup>-null mutant mice and their respective wild-type controls exposed to hypobaric hypoxia for 72 h. These findings confirm the previous observation that absence of the gp91<jats:sup>phox<\/jats:sup>component of the phagocytic NADPH oxidase does not alter the O<jats:sub>2<\/jats:sub>-sensing mechanism of the carotid body. However, absence of the p47<jats:sup>phox<\/jats:sup>component significantly potentiates ventilatory and chemoreceptor responses to hypoxia. O<jats:sub>2<\/jats:sub>sensing in EPO-producing cells of the kidney appears to be independent of the gp91<jats:sup>phox<\/jats:sup>and p47<jats:sup>phox<\/jats:sup>components of the phagocytic NADPH oxidase.<\/jats:p>","DOI":"10.1152\/japplphysiol.00564.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:42:01Z","timestamp":1425415321000},"page":"1357-1364","source":"Crossref","is-referenced-by-count":36,"title":["Role of components of the phagocytic NADPH oxidase in oxygen sensing"],"prefix":"10.1152","volume":"93","author":[{"given":"K. A.","family":"Sanders","sequence":"first","affiliation":[{"name":"Medical Service, Department of Veterans Affairs Medical Center, and Departments of"},{"name":"Internal Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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