{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T18:10:05Z","timestamp":1747764605006,"version":"3.41.0"},"reference-count":39,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2002,12,1]]},"abstract":"<jats:p>We tested the hypothesis that the ability of coronary arteries to withstand functional damage from superoxide (O[Formula: see text]) is altered by exposure of the arteries to a physiological concentration of \u03b2-estradiol. Female porcine coronary arterial rings were incubated in an O<jats:sub>2<\/jats:sub>-CO<jats:sub>2<\/jats:sub>incubator, under normoxic conditions, at 37\u00b0C for 22\u201324 h. Arteries were then placed in baths containing a physiological salt solution at 37\u00b0C with 95% O<jats:sub>2<\/jats:sub>-5% CO<jats:sub>2<\/jats:sub>for isometric force recordings. In rings from 14 female pigs, vasorelaxation to A-23187 and diethylamine-NONOate (DEA-NONOate) was determined with and without prior 15-min exposure to 400 \u03bcM pyrogallol. Sensitivity (\u2212logM ED<jats:sub>50<\/jats:sub>) and maximum relaxation to A-23187, but not DEA-NONOate, were significantly impaired by exposure to pyrogallol (pyrogallol treated: 7.39 \u00b1 0.09, 82 \u00b1 5%; control: 7.76 \u00b1 0.11, 99 \u00b1 1%, means \u00b1 SE; P &lt; 0.01 and P &lt; 0.05, respectively). This effect was attenuated by concurrent exposure to equimolar ascorbate. Arterial rings from 12 separate female pigs were incubated for 22\u201324 h with or without 1 nM \u03b2-estradiol before pyrogallol exposure. \u03b2-Estradiol significantly enhanced arterial sensitivity to A-23187 and prevented pyrogallol impairment without affecting DEA-NONOate responses. Therefore, superoxide-mediated endothelial damage and impaired endothelium-dependent relaxation of coronary arteries are prevented by overnight exposure of the arteries to a physiological concentration of \u03b2-estradiol.<\/jats:p>","DOI":"10.1152\/japplphysiol.00615.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:42:53Z","timestamp":1425415373000},"page":"1952-1958","source":"Crossref","is-referenced-by-count":6,"title":["O 2 \u2212 -mediated impairment of coronary arterial relaxation is prevented by overnight treatment with 1 nM \u03b2-estradiol"],"prefix":"10.1152","volume":"93","author":[{"given":"David R.","family":"Bell","sequence":"first","affiliation":[{"name":"Department of Cellular and Integrative Physiology, Indiana University, School of Medicine, Fort Wayne, Indiana 46805"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen E.","family":"Gochenaur","sequence":"additional","affiliation":[{"name":"Department 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