{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:40:52Z","timestamp":1790484052101,"version":"4.1.0"},"reference-count":47,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science (JSPS)","doi-asserted-by":"publisher","award":["17K19897"],"award-info":[{"award-number":["17K19897"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science (JSPS)","doi-asserted-by":"publisher","award":["16H0324"],"award-info":[{"award-number":["16H0324"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science (JSPS)","doi-asserted-by":"publisher","award":["15K12665"],"award-info":[{"award-number":["15K12665"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science (JSPS)","doi-asserted-by":"publisher","award":["23650403"],"award-info":[{"award-number":["23650403"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yamaha motor foundation for sports"},{"DOI":"10.13039\/100008732","name":"Uehara Memorial foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Sasakawa scientific research grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2019,3,1]]},"abstract":"<jats:p>Heat stress promotes intramyocyte calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) accumulation via transient receptor potential vanilloid 1 (TRPV1) channels. We tested the hypothesis that muscle contractile activity concomitant with heat stress would accelerate the increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>via TRPV1, further impairing [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>homeostasis. Spinotrapezius muscles of adult Wistar rats were exteriorized in vivo and loaded with the fluorescent Ca<jats:sup>2+<\/jats:sup>probe fura 2-AM. Heat stress (muscle surface temperature 40\u00b0C) was used as TRPV1 activator. An isometric contraction (100 Hz, 5\u201310 V, 30 s) was induced electrically concomitant with heat stress. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was determined for 20 min using in vivo fluorescence microscopy, and the phosphorylation response of TRPV1 was determined by Western blotting. Heat stress induced a significant [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>increase of 18.5\u2009\u00b1\u20098.1% at 20 min and TRPV1 phosphorylation (+231%), which was inhibited by addition of the TRPV1 inhibitor (capsazepine). However, contrary to expectations, the heat stress and isometric contraction condition almost completely inhibited TRPV1 phosphorylation and the consequent [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>elevation (&lt;2.8% accumulation during heat stress, P &gt; 0.05). In conclusion, this in vivo physiological model demonstrated that isometric muscle contraction(s) can suppress the phosphorylation response of TRPV1 and maintain [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>homeostasis during heat stress.<\/jats:p><jats:p>NEW &amp; NOTEWORTHY This investigation is the first document the dynamics of intramyocyte calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) increase in the myoplasm of skeletal muscle fibers in response to heat stress where the muscle blood flow is preserved. Heat stress at 40\u00b0C drives a myoplasmic [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>accumulation in concert with transient receptor potential vanilloid 1 (TRPV1) phosphorylation. However, muscle contraction caused TRPV1 channel deactivation by dephosphorylation of TRPV1. TRPV1 inactivation via isometric contraction(s) permits maintenance of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>homeostasis even under high imposed muscle temperature.<\/jats:p>","DOI":"10.1152\/japplphysiol.00668.2018","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T18:29:54Z","timestamp":1548354594000},"page":"691-698","source":"Crossref","is-referenced-by-count":19,"title":["Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions"],"prefix":"10.1152","volume":"126","author":[{"given":"Ryo","family":"Ikegami","sequence":"first","affiliation":[{"name":"Department of Engineering Science, Bioscience, and Technology Program, The University of Electro-Communications, Chofugaoka, Chofu, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Eshima","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative 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