{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:10:47Z","timestamp":1791216647721,"version":"4.1.0"},"reference-count":51,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:p>We examine the influence of the cytosolic and membrane-bound contents of carbonic anhydrase (CA; CAII, CAIII, CAIV, and CAXIV) and the muscle content of proteins involved in lactate and proton transport [monocarboxylate transporter (MCT) 1, MCT4, and Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>exchanger 1 (NHE1)] on work capacity during supramaximal exercise. Eight healthy, sedentary subjects performed exercises at 120% of the work rate corresponding to maximal oxygen uptake (\u1e86<jats:sub>max<\/jats:sub>) until exhaustion in placebo (Con) and metabolic alkalosis (Alk) conditions. The total (W<jats:sub>tot<\/jats:sub>) and supramaximal work performed (W<jats:sub>sup<\/jats:sub>) was measured. Muscle biopsies were obtained before and immediately after standardized exercises (se) at 120% \u1e86<jats:sub>max<\/jats:sub>in both conditions to determine the content of the targeted proteins, the decrease in muscle pH (\u0394pH<jats:sub>m<\/jats:sub>), and the muscle lactate accumulation ([Lac]<jats:sub>m<\/jats:sub>) per joule of W<jats:sub>sup<\/jats:sub>(\u0394pH<jats:sub>m<\/jats:sub>\/W<jats:sub>sup-se<\/jats:sub>and \u0394[Lac]<jats:sub>m<\/jats:sub>\/W<jats:sub>sup-se<\/jats:sub>, respectively) and the dynamic buffer capacity. In Con, W<jats:sub>sup<\/jats:sub>was negatively correlated with \u0394pH<jats:sub>m<\/jats:sub>\/W<jats:sub>sup-se<\/jats:sub>, positively correlated with \u0394[Lac]<jats:sub>m<\/jats:sub>\/W<jats:sub>sup-se<\/jats:sub>and MCT1, and tended to be positively correlated with MCT4 and NHE1. CAII + CAIII were correlated positively with \u0394pH<jats:sub>m<\/jats:sub>\/W<jats:sub>sup-se<\/jats:sub>and negatively with \u0394[Lac]<jats:sub>m<\/jats:sub>\/W<jats:sub>sup-se<\/jats:sub>, while CAIV was positively related to W<jats:sub>tot<\/jats:sub>. The changes in W<jats:sub>sup<\/jats:sub>with Alk were correlated positively with those in dynamic buffer capacity and negatively with W<jats:sub>sup<\/jats:sub>in Con. Performance improvement with Alk was greater in subjects having a low content of proteins involved in pH regulation and lactate\/proton transport. These results show the importance of pH regulating mechanisms and lactate\/proton transport on work capacity and the role of the CA to delay decrease in pH<jats:sub>m<\/jats:sub>and accumulation in [Lac]<jats:sub>m<\/jats:sub>during supramaximal exercise in humans.<\/jats:p>","DOI":"10.1152\/japplphysiol.00691.2006","type":"journal-article","created":{"date-parts":[[2007,2,9]],"date-time":"2007-02-09T02:05:05Z","timestamp":1170986705000},"page":"1936-1944","source":"Crossref","is-referenced-by-count":127,"title":["Importance of pH regulation and lactate\/H<sup>+<\/sup>transport capacity for work production during supramaximal exercise in 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