{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T20:56:37Z","timestamp":1790369797917,"version":"4.1.0"},"reference-count":73,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/100000090","name":"DOD | Congressionally Directed Medical Research Programs (CDMRP)","doi-asserted-by":"publisher","award":["SC140038"],"award-info":[{"award-number":["SC140038"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005191","name":"Craig H. Neilsen Foundation","doi-asserted-by":"publisher","award":["338432"],"award-info":[{"award-number":["338432"]}],"id":[{"id":"10.13039\/100005191","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH (PO1)","award":["NS 055976"],"award-info":[{"award-number":["NS 055976"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2018,9,1]]},"abstract":"<jats:p>\n                    Spinal cord injury (SCI) at the level of cervical segments often results in life-threatening respiratory complications and requires long-term mechanical ventilator assistance. Thus restoring diaphragm activity and regaining voluntary control of breathing are the primary clinical goals for patients with respiratory dysfunction following cervical SCI. Epidural stimulation (EDS) is a promising strategy that has been explored extensively for nonrespiratory functions and to a limited extent within the respiratory system. The goal of the present study is to assess the potential for EDS at the location of the phrenic nucleus (C\n                    <jats:sub>3<\/jats:sub>\n                    \u2013C\n                    <jats:sub>5<\/jats:sub>\n                    ) innervating the diaphragm: the main inspiratory muscle following complete C\n                    <jats:sub>1<\/jats:sub>\n                    cervical transection. To avoid the suppressive effect of anesthesia, all experiments were performed in decerebrate, C\n                    <jats:sub>1<\/jats:sub>\n                    cervical transection, unanesthetized, nonparalyzed ( n = 13) and paralyzed ( n = 7) animals. Our results show that C\n                    <jats:sub>4<\/jats:sub>\n                    segment was the most responsive to EDS and required the lowest threshold of current intensity, affecting tracheal pressure and phrenic nerve responses. High-frequency (200\u2013300 Hz) EDS applied over C\n                    <jats:sub>4<\/jats:sub>\n                    segment (C\n                    <jats:sub>4<\/jats:sub>\n                    -EDS) was able to maintain breathing with normal end-tidal CO\n                    <jats:sub>2<\/jats:sub>\n                    level and raise blood pressure. In addition, 100\u2013300 Hz of C\n                    <jats:sub>4<\/jats:sub>\n                    -EDS showed time- and frequency-dependent changes (short-term facilitation) of evoked phrenic nerve responses that may serve as a target mechanism for pacing of phrenic motor circuits. The present work provides the first report of successful EDS at the level of phrenic nucleus in a complete SCI animal model and offers insight into the potential therapeutic application in patients with high cervical SCI.\n                  <\/jats:p>\n                  <jats:p>NEW &amp; NOTEWORTHY The present work offers the first demonstration of successful life-supporting breathing paced by epidural stimulation (EDS) at the level of the phrenic nucleus, following a complete spinal cord injury in unanesthetized, decerebrate rats. Moreover, our experiments showed time- and frequency-dependent changes of evoked phrenic nerve activity during EDS that may serve as a target mechanism for pacing spinal phrenic motor networks.<\/jats:p>","DOI":"10.1152\/japplphysiol.00895.2017","type":"journal-article","created":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T13:58:53Z","timestamp":1526565533000},"page":"687-696","source":"Crossref","is-referenced-by-count":22,"title":["Paced breathing and phrenic nerve responses evoked by epidural stimulation following complete high cervical spinal cord injury in rats"],"prefix":"10.1152","volume":"125","author":[{"given":"Tatiana","family":"Bezdudnaya","sequence":"first","affiliation":[{"name":"Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Lane","sequence":"additional","affiliation":[{"name":"Department of Neurobiology and Anatomy, 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