{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T05:29:21Z","timestamp":1761197361924},"reference-count":61,"publisher":"American Physiological Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2016,5,15]]},"abstract":"<jats:p> The intrinsic activating factors that induce transcription of heat shock protein 72 (HSP72) in skeletal muscle following exercise remain unclear. We hypothesized that the cytosolic Ca<jats:sup>2+<\/jats:sup> transient that occurs with depolarization is a determinant. We utilized intact, single skeletal muscle fibers from Xenopus laevis to test the role of the cytosolic Ca<jats:sup>2+<\/jats:sup> transient and several other exercise-related factors (fatigue, hypoxia, AMP kinase, and cross-bridge cycling) on the activation of HSP72 transcription. HSP72 and HSP60 mRNA levels were assessed with real-time quantitative PCR; cytosolic Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub>) was assessed with fura-2. Both fatiguing and nonfatiguing contractions resulted in a significant increase in HSP72 mRNA. As expected, peak [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub> remained tightly coupled with peak developed tension in contracting fibers. Pretreatment with N-benzyl- p-toluene sulfonamide (BTS) resulted in depressed peak developed tension with stimulation, while peak [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub> remained largely unchanged from control values. Despite excitation-contraction uncoupling, BTS-treated fibers displayed a significant increase in HSP72 mRNA. Treatment of fibers with hypoxia (Po<jats:sub>2<\/jats:sub>: &lt;3 mmHg) or AMP kinase activation had no effect on HSP72 mRNA levels. These results suggest that the intermittent cytosolic Ca<jats:sup>2+<\/jats:sup> transient that occurs with skeletal muscle depolarization provides a sufficient activating stimulus for HSP72 transcription. Metabolic or mechanical factors associated with fatigue development and cross-bridge cycling likely play a more limited role. <\/jats:p>","DOI":"10.1152\/japplphysiol.01060.2015","type":"journal-article","created":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T04:19:22Z","timestamp":1455250762000},"page":"1260-1266","source":"Crossref","is-referenced-by-count":6,"title":["Cytosolic calcium transients are a determinant of contraction-induced HSP72 transcription in single skeletal muscle fibers"],"prefix":"10.1152","volume":"120","author":[{"given":"Creed M.","family":"Stary","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California, San Diego, La Jolla, California; and"},{"name":"Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, Stanford, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael 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