{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:52:28Z","timestamp":1792443148646,"version":"4.5.0"},"reference-count":66,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>\n                    Defects in mitochondrial dynamics, the processes of fission, fusion, and mitochondrial autophagy, may contribute to metabolic disease including type 2 diabetes. Dynamin-related protein-1 (Drp1) is a GTPase protein that plays a central role in mitochondrial fission. We hypothesized that aerobic exercise training would decrease Drp1 Ser\n                    <jats:sup>616<\/jats:sup>\n                    phosphorylation and increase fat oxidation and insulin sensitivity in obese (body mass index: 34.6 \u00b1 0.8 kg\/m\n                    <jats:sup>2<\/jats:sup>\n                    ) insulin-resistant adults. Seventeen subjects performed supervised exercise for 60 min\/day, 5 days\/wk at 80\u201385% of maximal heart rate for 12 wk. Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp, and fat oxidation was determined by indirect calorimetry. Skeletal muscle biopsies were obtained from the vastus lateralis muscle before and after the 12-wk program. The exercise intervention increased insulin sensitivity 2.1 \u00b1 0.2-fold ( P &lt; 0.01) and fat oxidation 1.3 \u00b1 0.3-fold ( P &lt; 0.01). Phosphorylation of Drp1 at Ser\n                    <jats:sup>616<\/jats:sup>\n                    was decreased (pre vs. post: 0.81 \u00b1 0.15 vs. 0.58 \u00b1 0.14 arbitrary units; P &lt; 0.05) following the intervention. Furthermore, reductions in Drp1 Ser\n                    <jats:sup>616<\/jats:sup>\n                    phosphorylation were negatively correlated with increases in fat oxidation ( r = \u22120.58; P &lt; 0.05) and insulin sensitivity (rho = \u22120.52; P &lt; 0.05). We also examined expression of genes related to mitochondrial dynamics. Dynamin1-like protein (DNM1L; P &lt; 0.01), the gene that codes for Drp1, and Optic atrophy 1 (OPA1; P = 0.05) were significantly upregulated following the intervention, while there was a trend towards an increase in expression of both mitofusin protein MFN1 ( P = 0.08) and MFN2 ( P = 0.07). These are the first data to suggest that lifestyle-mediated improvements in substrate metabolism and insulin sensitivity in obese insulin-resistant adults may be regulated through decreased activation of the mitochondrial fission protein Drp1.\n                  <\/jats:p>","DOI":"10.1152\/japplphysiol.01064.2013","type":"journal-article","created":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T04:20:30Z","timestamp":1403238030000},"page":"239-245","source":"Crossref","is-referenced-by-count":132,"title":["Exercise training decreases activation of the mitochondrial fission protein dynamin-related protein-1 in insulin-resistant human skeletal muscle"],"prefix":"10.1152","volume":"117","author":[{"given":"Ciaran E.","family":"Fealy","sequence":"first","affiliation":[{"name":"Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio;"},{"name":"Department of Biomedical Sciences, Kent State University, Kent, 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