{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:12:49Z","timestamp":1773292369457,"version":"3.50.1"},"reference-count":58,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p>The O<jats:sub>2<\/jats:sub>sensitivity of dissociated type I cells from rat carotid body increases with age until \u223c14\u201316 days. Hypoxia-induced depolarization appears to be mediated by an O<jats:sub>2<\/jats:sub>-sensitive K<jats:sup>+<\/jats:sup>current, but other K<jats:sup>+<\/jats:sup>currents may modulate depolarization. We hypothesized that membrane potential may be stabilized in newborn type I cells by human ether-a-go-go-related gene (HERG)-like K<jats:sup>+<\/jats:sup>currents that inhibit hypoxia-induced depolarization and that a decrease in this current with age could underlie, in part, the developmental increase in type I cell depolarization response to hypoxia. In dissociated type I cells from 0- to 1- and 11- to 16-day-old rats, using perforated patch-clamp and 70 mM K<jats:sup>+<\/jats:sup>extracellular solution, we measured repolarization-induced inward K<jats:sup>+<\/jats:sup>tail currents in the absence and presence of E-4031, a specific HERG channel blocker. This allowed isolation of the E-4031-sensitive HERG-like current. E-4031-sensitive peak currents in type I cells from 0- to- 1-day-old rats were 2.5-fold larger than in cells from 11- to 16-day-old rats. E-4031-sensitive current density in newborn type I cells was twofold greater than in cells from 11- to 16-day-old rats. Under current clamp conditions, E-4031 enhanced hypoxia-induced depolarization in type I cells from 0- to- 1-day-old but not 11- to 16-day-old rats. With use of fura 2 to measure intracellular Ca<jats:sup>2+<\/jats:sup>, E-4031 increased the cytosolic Ca<jats:sup>2+<\/jats:sup>concentration response to anoxia in cells from 0- to- 1-day-old but not cells from 11- to 16-day-old rats. E-4031-sensitive K<jats:sup>+<\/jats:sup>currents are present in newborn carotid body type I cells and decline with age. These findings are consistent with a role for E-4031-sensitive K<jats:sup>+<\/jats:sup>current, and possibly HERG-like K<jats:sup>+<\/jats:sup>currents, in the type I cell hypoxia response maturation.<\/jats:p>","DOI":"10.1152\/japplphysiol.01254.2003","type":"journal-article","created":{"date-parts":[[2005,3,16]],"date-time":"2005-03-16T05:32:59Z","timestamp":1110951179000},"page":"1469-1477","source":"Crossref","is-referenced-by-count":15,"title":["Postnatal development of E-4031-sensitive potassium current in rat carotid chemoreceptor cells"],"prefix":"10.1152","volume":"98","author":[{"given":"Insook","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen M.","family":"Boyle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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