{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:23:37Z","timestamp":1790839417143,"version":"4.1.0"},"reference-count":35,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2007,4]]},"abstract":"\n Skeletal muscle contractility and myosin function decline following ovariectomy in mature female mice. In the present study we tested the hypothesis that estradiol replacement can reverse those declines. Four-month-old female C57BL\/6 mice ( n = 69) were ovariectomized (OVX) or sham operated. Some mice were treated immediately with placebo or 17\u03b2-estradiol (OVX + E\n 2<\/jats:sub>\n ) while other mice were treated 30 days postsurgery. Thirty or sixty days postsurgery, soleus muscles were assessed in vitro for contractile function and susceptibility to eccentric contraction-induced injury. Myosin structural dynamics was analyzed in extensor digitorum longus (EDL) muscles by electron paramagnetic resonance spectroscopy. Maximal isometric tetanic force was affected by estradiol status ( P < 0.001) being \u223c10% less in soleus muscles from OVX compared with sham-operated mice [168 mN (SD 16.7) vs. 180 mN (SD 14.4)] and was restored in OVX + E\n 2<\/jats:sub>\n mice [187 mN (SD 17.6)]. The fraction of strong-binding myosin during contraction was also affected ( P = 0.045) and was \u223c15% lower in EDL muscles from OVX compared with OVX + E\n 2<\/jats:sub>\n mice [0.263 (SD 0.034) vs. 0.311 (SD 0.022)]. Plasma estradiol levels were correlated with maximal isometric tetanic force ( r = 0.458; P < 0.001) and active stiffness ( r = 0.329; P = 0.044), indicating that circulating estradiol influenced muscle and myosin function. Estradiol was not effective in protecting muscle against an acute eccentric contraction-induced injury ( P \u2265 0.401) but did restore ovariectomy-induced increases in muscle wet mass caused by fluid accumulation. Collectively, estradiol had a beneficial effect on female mouse skeletal muscle.\n <\/jats:p>","DOI":"10.1152\/japplphysiol.01305.2006","type":"journal-article","created":{"date-parts":[[2007,1,11]],"date-time":"2007-01-11T20:34:46Z","timestamp":1168547686000},"page":"1387-1393","source":"Crossref","is-referenced-by-count":176,"title":["Estradiol replacement reverses ovariectomy-induced muscle contractile and myosin dysfunction in mature female mice"],"prefix":"10.1152","volume":"102","author":[{"given":"Amy L.","family":"Moran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel M.","family":"Landisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon 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