{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:36:05Z","timestamp":1792290965062,"version":"4.4.0"},"reference-count":45,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:p>\n                    Airway exposure to zinc dust and zinc-containing ambient particulates can cause symptoms of airway irritation and inflammation, but the underlying molecular and cellular mechanisms are largely unknown. Transient receptor potential A1 (TRPA1) is selectively expressed in a subpopulation of pulmonary C-fiber afferents and has been considered as a major irritant sensor in the lung and airways. Using whole cell patch-clamp recording and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    imaging, we have demonstrated that application of ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    concentration dependently evoked inward current and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transient in isolated vagal pulmonary sensory neurons; both responses were almost completely inhibited after pretreatment with AP18, a specific TRPA1 antagonist. In anesthetized spontaneously breathing animals, intratracheal instillation of ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    (2 mM, 25 \u03bcl) induced pronounced respiratory depression in wild-type mice, and the effect was essentially absent in TRPA1-deficient mice. In addition, our study showed that two other heavy metals, cadmium and copper, also stimulated pulmonary sensory neurons via a direct activation of TRPA1. In summary, our results suggest that activation of TRPA1 in pulmonary C-fiber sensory nerves may contribute to the respiratory toxicity induced by airway exposure to these three heavy metals.\n                  <\/jats:p>","DOI":"10.1152\/japplphysiol.01371.2009","type":"journal-article","created":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T21:51:04Z","timestamp":1265320264000},"page":"891-897","source":"Crossref","is-referenced-by-count":68,"title":["Heavy metals zinc, cadmium, and copper stimulate pulmonary sensory neurons via direct activation of TRPA1"],"prefix":"10.1152","volume":"108","author":[{"given":"Qihai","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruei-Lung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Kentucky Medical Center, Lexington, 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