{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:53:22Z","timestamp":1761058402093},"reference-count":42,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p>Intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) governs the contractile status of arteriolar smooth muscle cells (SMC). Although studied in vitro, little is known of SMC [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>dynamics during the local control of blood flow. We tested the hypothesis that the rise and fall of SMC [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>underlies arteriolar constriction and dilation in vivo. Aparenchymal segments of second-order arterioles (diameter 35 \u00b1 2 \u03bcm) were prepared in the superfused cheek pouch of anesthetized hamsters ( n = 18) and perifused with the ratiometric dye fura PE-3 (AM) to load SMC (1 \u03bcM, 20 min). Resting SMC [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was 406 \u00b1 37 nM. Elevating superfusate O<jats:sub>2<\/jats:sub>from 0 to 21% produced constriction (11 \u00b1 2 \u03bcm) that was unaffected by dye loading; [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>increased by 108 \u00b1 53 nM ( n = 6, P &lt; 0.05). Cycling of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>during vasomotion (amplitude, 150 \u00b1 53 nM; n = 4) preceded corresponding diameter changes (7 \u00b1 1 \u03bcm) by \u223c2 s. Microiontophoresis (1 \u03bcm pipette tip; 1 \u03bcA, 1 s) of phenylephrine (PE) transiently increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>by 479 \u00b1 64 nM ( n = 8, P &lt; 0.05) with constriction (26 \u00b1 3 \u03bcm). Flushing blood from the lumen with saline increased fluorescence at 510 nm by \u223c45% during excitation at both 340 and 380 nm with no difference in resting [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, diameter or respective responses to PE ( n = 7). Acetylcholine microiontophoresis (1 \u03bcA, 1 s) transiently reduced resting SMC [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>by 131 \u00b1 21 nM ( n = 6, P &lt; 0.05) with vasodilation (17 \u00b1 1 \u03bcm). Superfusion of sodium nitroprusside (10 \u03bcM) transiently reduced SMC [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>by 124 \u00b1 18 nM ( n = 6, P &lt; 0.05), whereas dilation (23 \u00b1 5 \u03bcm) was sustained. Resolution of arteriolar SMC [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in vivo discriminates key signaling events that govern the local control of tissue blood flow.<\/jats:p>","DOI":"10.1152\/japplphysiol.01634.2005","type":"journal-article","created":{"date-parts":[[2006,2,3]],"date-time":"2006-02-03T01:48:58Z","timestamp":1138931338000},"page":"307-315","source":"Crossref","is-referenced-by-count":27,"title":["Arteriolar smooth muscle Ca<sup>2+<\/sup>dynamics during blood flow control in hamster cheek pouch"],"prefix":"10.1152","volume":"101","author":[{"given":"Johan Fredrik","family":"Brekke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William F.","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven 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