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Experiments here have assessed the relative content of ryanodine receptor-gated stores in CA1 neurons by measuring cytoplasmic Ca<jats:sup>2+<\/jats:sup>increases induced by caffeine. These measurements were performed on CA1 neurons, in slice, from normal gerbils, and compared with responses from this same population of neurons 54\u201360 h after animals had undergone a standard ischemic insult: 5-min bilateral occlusion of the carotid arteries. The mean amplitude of responses in the postischemic population were less than one-third of those in control or sham-operated animals, and 35% of the neurons from postischemic animals showed very small responses that were \u223c10% of the control population mean. Refilling of these stores after caffeine challenges was also impaired in postischemic neurons. These observations are consistent with our earlier finding that voltage-gated influx is sharply reduced in postischemic in CA1 neurons and the hypothesis that the resulting depletion in endosomal Ca<jats:sup>2+<\/jats:sup>is an important cause of delayed neuronal death.<\/jats:p>","DOI":"10.1152\/jn.00015.2004","type":"journal-article","created":{"date-parts":[[2004,6,17]],"date-time":"2004-06-17T00:34:17Z","timestamp":1087432457000},"page":"2960-2967","source":"Crossref","is-referenced-by-count":12,"title":["Caffeine Releasable Stores of Ca<sup>2+<\/sup>Show Depletion Prior to the Final Steps in Delayed CA1 Neuronal Death"],"prefix":"10.1152","volume":"92","author":[{"given":"Hong","family":"Xing","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aryan","family":"Azimi-Zonooz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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