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To model sharp waves in a relatively large hippocampal circuitry in vitro, we developed thick (1 mm) mouse hippocampal slices by separating the dentate gyrus from the CA2\/CA1 areas while keeping the functional dentate gyrus-CA3-CA1 connections. We found that large amplitude (0.3\u20133 mV) sharp wave-like field potentials occurred spontaneously in the thick slices without extra ionic or pharmacological manipulation and they resemble closely electroencephalographic sharp waves with respect to waveform, regional initiation, pharmacological manipulations, and intracellular correlates. Through measuring tissue O<jats:sub>2<\/jats:sub>, K<jats:sup>+<\/jats:sup>, and synaptic and single cell activities, we verified that the sharp wave-like potentials are not a consequence of anoxia, nonspecific elevation of extracellular K<jats:sup>+<\/jats:sup>and dissection-related tissue damage. Our data suggest that a subtle but crucial increase in the CA3 glutamatergic activity effectively recruits a population of neurons thus responsible for the generation of the sharp wave-like spontaneous field potentials in isolated hippocampal circuitry.<\/jats:p>","DOI":"10.1152\/jn.00086.2005","type":"journal-article","created":{"date-parts":[[2005,3,17]],"date-time":"2005-03-17T01:24:47Z","timestamp":1111022687000},"page":"741-753","source":"Crossref","is-referenced-by-count":45,"title":["An In Vitro Model of Hippocampal Sharp Waves: Regional Initiation and Intracellular Correlates"],"prefix":"10.1152","volume":"94","author":[{"given":"Chiping","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjan 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