{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:47:33Z","timestamp":1790700453518,"version":"4.1.0"},"reference-count":70,"publisher":"American Physiological Society","issue":"2","funder":[{"name":"NIH-NIDA"},{"DOI":"10.13039\/100000001","name":"National Science Foundation (NSF)","doi-asserted-by":"publisher","award":["IOS-1355034"],"award-info":[{"award-number":["IOS-1355034"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Latham Trust Fund"},{"DOI":"10.13039\/100001391","name":"Whitehall Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001391","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH","award":["GM08016"],"award-info":[{"award-number":["GM08016"]}]},{"name":"NIH","award":["EY24625"],"award-info":[{"award-number":["EY24625"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:p>The endocannabinoid (eCB) signaling system has been functionally implicated in many brain regions. Our understanding of the role of cannabinoid receptor type 1 (CB<jats:sub>1<\/jats:sub>) in olfactory processing remains limited. Cannabinoid signaling is involved in regulating glomerular activity in the main olfactory bulb (MOB). However, the cannabinoid-related circuitry of inputs to mitral cells in the MOB has not been fully determined. Using anatomical and functional approaches we have explored this question. CB<jats:sub>1<\/jats:sub>was present in periglomerular processes of a GAD65-positive subpopulation of interneurons but not in mitral cells. We detected eCBs in the mouse MOB as well as the expression of CB<jats:sub>1<\/jats:sub>and other genes associated with cannabinoid signaling in the MOB. Patch-clamp electrophysiology demonstrated that CB<jats:sub>1<\/jats:sub>agonists activated mitral cells and evoked an inward current, while CB<jats:sub>1<\/jats:sub>antagonists reduced firing and evoked an outward current. CB<jats:sub>1<\/jats:sub>effects on mitral cells were absent in subglomerular slices in which the olfactory nerve layer and glomerular layer were removed, suggesting the glomerular layer as the site of CB<jats:sub>1<\/jats:sub>action. We previously observed that GABAergic periglomerular cells show the inverse response pattern to CB<jats:sub>1<\/jats:sub>activation compared with mitral cells, suggesting that CB<jats:sub>1<\/jats:sub>indirectly regulates mitral cell activity as a result of cellular activation of glomerular GABAergic processes . This hypothesis was supported by the finding that cannabinoids modulated synaptic transmission to mitral cells. We conclude that CB<jats:sub>1<\/jats:sub>directly regulates GABAergic processes in the glomerular layer to control GABA release and, in turn, regulates mitral cell activity with potential effects on olfactory threshold and behavior.<\/jats:p><jats:p>NEW &amp; NOTEWORTHY Cannabinoid signaling with cannabinoid receptor type 1 (CB<jats:sub>1<\/jats:sub>) is involved in the regulation of glomerular activity in the main olfactory bulb (MOB). We detected endocannabinoids in the mouse MOB. CB<jats:sub>1<\/jats:sub>was present in periglomerular processes of a GAD65-positive subpopulation of interneurons. CB<jats:sub>1<\/jats:sub>agonists activated mitral cells. CB<jats:sub>1<\/jats:sub>directly regulates GABAergic processes to control GABA release and, in turn, regulates mitral cell activity with potential effects on olfactory threshold and behavior.<\/jats:p>","DOI":"10.1152\/jn.00100.2018","type":"journal-article","created":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T11:53:07Z","timestamp":1560945187000},"page":"749-759","source":"Crossref","is-referenced-by-count":20,"title":["Cannabinoid receptor-mediated modulation of inhibitory inputs to mitral cells in the main olfactory bulb"],"prefix":"10.1152","volume":"122","author":[{"given":"Ze-Jun","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anatomy, Howard University College of Medicine, Washington, District of Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry Shu-Jung","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Psychology, National Cheng Kung University, Tainan, 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