{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T07:51:45Z","timestamp":1760255505485,"version":"3.37.3"},"reference-count":49,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/501100000024","name":"Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de recherche en sant\u00e9 du Canada)","doi-asserted-by":"publisher","award":["MOP12192"],"award-info":[{"award-number":["MOP12192"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurophysiology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p> Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) is an endogenously found gasotransmitter that has been implicated in a variety of beneficial physiological functions. This study was performed to investigate the cellular mechanisms underlying actions of H<jats:sub>2<\/jats:sub>S previously observed in subfornical organ (SFO), where H<jats:sub>2<\/jats:sub>S acts to regulate blood pressure through a depolarization of the membrane and an overall increase in the excitability of SFO neurons. We used whole cell patch-clamp electrophysiology in the voltage-clamp configuration to analyze the effect of 1 mM NaHS, an H<jats:sub>2<\/jats:sub>S donor, on voltage-gated potassium, sodium, and calcium currents. We observed no effect of NaHS on potassium currents; however, both voltage-gated sodium currents (persistent and transient) and the N-type calcium current had a depolarized activation curve and an enhanced peak-induced current in response to a series of voltage-step and ramp protocols run in the control and NaHS conditions. These effects were not responsible for the previously observed depolarization of the membrane potential, as depolarizing effects of H<jats:sub>2<\/jats:sub>S were still observed following block of these conductances with tetrodotoxin (5 \u03bcM) and \u03c9-conotoxin-GVIA (100 nM). Our studies are the first to investigate the effect of H<jats:sub>2<\/jats:sub>S on a variety of voltage-gated conductances in a single brain area, and although they do not explain mechanisms underlying the depolarizing actions of H<jats:sub>2<\/jats:sub>S on SFO neurons, they provide evidence of potential mechanisms through which this gasotransmitter influences the excitability of neurons in this important brain area as a consequence of the modulation of multiple ion channels. <\/jats:p>","DOI":"10.1152\/jn.00252.2015","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T22:50:06Z","timestamp":1437000606000},"page":"1641-1651","source":"Crossref","is-referenced-by-count":15,"title":["Actions of a hydrogen sulfide donor (NaHS) on transient sodium, persistent sodium, and voltage-gated calcium currents in neurons of the subfornical 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